Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3076737..3077424 Replicon chromosome
Accession NZ_CP041816
Organism Mycobacterium tuberculosis strain SEA00042P6C4

Toxin (Protein)


Gene name vapC Uniprot ID G0TF65
Locus tag FPJ93_RS14440 Protein ID WP_003414064.1
Coordinates 3077029..3077424 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag FPJ93_RS14435 Protein ID WP_003414061.1
Coordinates 3076737..3077003 (-) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ93_RS14410 3072386..3073288 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
FPJ93_RS14415 3073357..3074109 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
FPJ93_RS14420 3074353..3074628 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
FPJ93_RS14425 3074625..3076237 - 1613 Protein_2846 type I restriction-modification system subunit M -
FPJ93_RS14430 3076324..3076740 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 -
FPJ93_RS14435 3076737..3077003 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
FPJ93_RS14440 3077029..3077424 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin Toxin
FPJ93_RS14445 3077421..3077690 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
FPJ93_RS14450 3077700..3078794 - 1095 WP_003900565.1 restriction endonuclease subunit S -
FPJ93_RS14455 3078791..3079210 - 420 WP_003414070.1 winged helix-turn-helix domain-containing protein -
FPJ93_RS14460 3079284..3079763 - 480 WP_003414073.1 dihydrofolate reductase -
FPJ93_RS14465 3079834..3080634 - 801 WP_003911953.1 thymidylate synthase -
FPJ93_RS14470 3080790..3081527 + 738 WP_003414079.1 dienelactone hydrolase family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-125)

Antitoxin

(16-56)


Sequences


Toxin        


Download         Length: 132 a.a.        Molecular weight: 14340.04 Da        Isoelectric Point: 4.7145

>T130991 WP_003414064.1 NZ_CP041816:c3077424-3077029 [Mycobacterium tuberculosis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Download         Length: 396 bp

>T130991 NZ_CP041816:c3077424-3077029 [Mycobacterium tuberculosis]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT130991 WP_003414061.1 NZ_CP041816:c3077003-3076737 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT130991 NZ_CP041816:c3077003-3076737 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB G0TF65


Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References