Detailed information of TA system TA130812

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3159613..3160217
• Replicon: chromosome
• Accession: NZ_CP041813
• Organism: Mycobacterium tuberculosis strain SEA07010354P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: FPJ96_RS14915
• Protein ID: WP_003414492.1
• Coordinates: 3159613..3160005 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A829CBY8
• Locus tag: FPJ96_RS14920
• Protein ID: WP_003414495.1
• Coordinates: 3160002..3160217 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ96_RS14885	3154763..3155551	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
FPJ96_RS14890	3155885..3156430	-	546	WP_014584866.1	DUF1802 family protein	-
FPJ96_RS14895	3156702..3157586	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
FPJ96_RS14900	3157589..3158476	-	888	WP_015630563.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
FPJ96_RS14905	3158781..3159326	-	546	WP_019284034.1	DUF1802 family protein	-
FPJ96_RS14910	3159323..3159592	-	270	WP_003414489.1	DUF2277 family protein	-
FPJ96_RS14915	3159613..3160005	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ96_RS14920	3160002..3160217	-	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FPJ96_RS14925	3160264..3161013	+	750	WP_003414497.1	enoyl-CoA hydratase	-
FPJ96_RS14930	3161092..3162174	-	1083	WP_003414499.1	ABC transporter ATP-binding protein	-
FPJ96_RS14935	3162167..3163477	-	1311	WP_003414501.1	ABC transporter substrate-binding protein	-
FPJ96_RS14940	3163480..3164307	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
FPJ96_RS14945	3164304..3165215	-	912	WP_003414505.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3127028..3160554	33526

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T130812 WP_003414492.1 NZ_CP041813:c3160005-3159613 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T130812 NZ_CP041813:c3160005-3159613 [Mycobacterium tuberculosis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7511.55 Da
• Isoelectric Point: 4.9732

>AT130812 WP_003414495.1 NZ_CP041813:c3160217-3160002 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT130812 NZ_CP041813:c3160217-3160002 [Mycobacterium tuberculosis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8

Antitoxin

• AlphaFold DB: A0A829CBY8