Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3133379..3133949 Replicon chromosome
Accession NZ_CP041813
Organism Mycobacterium tuberculosis strain SEA07010354P6C4

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag FPJ96_RS14770 Protein ID WP_003414166.1
Coordinates 3133379..3133735 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag FPJ96_RS14775 Protein ID WP_003901465.1
Coordinates 3133719..3133949 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ96_RS14750 3128831..3130519 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
FPJ96_RS14755 3130523..3130849 - 327 WP_003414157.1 hypothetical protein -
FPJ96_RS14760 3131022..3131609 + 588 WP_003914429.1 DUF3558 family protein -
FPJ96_RS14765 3131628..3133277 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
FPJ96_RS14770 3133379..3133735 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
FPJ96_RS14775 3133719..3133949 - 231 WP_003901465.1 antitoxin MazE Antitoxin
FPJ96_RS14780 3133992..3135035 - 1044 WP_016720604.1 DUF2293 domain-containing protein -
FPJ96_RS14785 3135226..3135501 + 276 WP_003911993.1 DUF1778 domain-containing protein -
FPJ96_RS14790 3135677..3135931 - 255 WP_155936531.1 hypothetical protein -
FPJ96_RS14795 3136079..3136471 + 393 WP_078397589.1 hypothetical protein -
FPJ96_RS14800 3136468..3136659 + 192 WP_003414184.1 hypothetical protein -
FPJ96_RS14805 3136723..3138011 + 1289 Protein_2922 transposase family protein -
FPJ96_RS14810 3138245..3138472 + 228 Protein_2923 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 3127028..3160554 33526


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T130810 WP_003414166.1 NZ_CP041813:c3133735-3133379 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T130810 NZ_CP041813:c3133735-3133379 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT130810 WP_003901465.1 NZ_CP041813:c3133949-3133719 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT130810 NZ_CP041813:c3133949-3133719 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References