Detailed information of TA system TA130781

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1246886..1247454
• Replicon: chromosome
• Accession: NZ_CP041813
• Organism: Mycobacterium tuberculosis strain SEA07010354P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: FPJ96_RS05885
• Protein ID: WP_003405865.1
• Coordinates: 1247080..1247454 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: FPJ96_RS05880
• Protein ID: WP_003405863.1
• Coordinates: 1246886..1247083 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ96_RS05860	1242927..1243565	-	639	WP_003898730.1	lipid droplet-associated protein	-
FPJ96_RS05865	1243655..1244662	+	1008	WP_015629829.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
FPJ96_RS05870	1244679..1245662	-	984	WP_003898731.1	hypothetical protein	-
FPJ96_RS05875	1245725..1246798	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
FPJ96_RS05880	1246886..1247083	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPJ96_RS05885	1247080..1247454	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ96_RS05890	1247657..1248355	+	699	WP_016719357.1	hypothetical protein	-
FPJ96_RS05895	1248473..1248658	+	186	WP_003901093.1	hypothetical protein	-
FPJ96_RS05900	1248585..1248860	-	276	WP_003405867.1	hypothetical protein	-
FPJ96_RS05905	1249103..1249426	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
FPJ96_RS05910	1249441..1250151	-	711	WP_003911399.1	hypothetical protein	-
FPJ96_RS05915	1250173..1250382	+	210	WP_003911400.1	hypothetical protein	-
FPJ96_RS05920	1250334..1251041	-	708	Protein_1166	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T130781 WP_003405865.1 NZ_CP041813:1247080-1247454 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T130781 NZ_CP041813:1247080-1247454 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT130781 WP_003405863.1 NZ_CP041813:1246886-1247083 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT130781 NZ_CP041813:1246886-1247083 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07