Detailed information of TA system TA130744

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2870273..2870904
• Replicon: chromosome
• Accession: NZ_CP041812
• Organism: Mycobacterium tuberculosis strain SEA07020250P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: FPJ97_RS13360
• Protein ID: WP_003413174.1
• Coordinates: 2870527..2870904 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: FPJ97_RS13355
• Protein ID: WP_003413167.1
• Coordinates: 2870273..2870530 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ97_RS13325	2866187..2866501	+	315	WP_009937839.1	hypothetical protein	-
FPJ97_RS13330	2866797..2868008	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
FPJ97_RS13335	2868135..2868794	+	660	WP_014585545.1	LppA family lipoprotein	-
FPJ97_RS13340	2868791..2869453	+	663	WP_031730273.1	hypothetical protein	-
FPJ97_RS13345	2869450..2869728	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
FPJ97_RS13350	2869821..2870234	+	414	WP_003413164.1	PIN domain nuclease	-
FPJ97_RS13355	2870273..2870530	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
FPJ97_RS13360	2870527..2870904	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ97_RS13365	2870920..2871294	-	375	WP_003413177.1	hypothetical protein	-
FPJ97_RS13370	2871394..2871789	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
FPJ97_RS13375	2871786..2872031	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
FPJ97_RS13380	2872442..2872861	-	420	WP_003413190.1	A24 family peptidase	-
FPJ97_RS13385	2872873..2873682	-	810	WP_003413193.1	shikimate dehydrogenase	-
FPJ97_RS13390	2873679..2874932	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
FPJ97_RS13395	2874925..2875437	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T130744 WP_003413174.1 NZ_CP041812:2870527-2870904 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T130744 NZ_CP041812:2870527-2870904 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT130744 WP_003413167.1 NZ_CP041812:2870273-2870530 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT130744 NZ_CP041812:2870273-2870530 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5