Detailed information of TA system TA130669

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2191171..2191874
• Replicon: chromosome
• Accession: NZ_CP041811
• Organism: Mycobacterium tuberculosis strain SEA08151P6C4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: FPJ98_RS10030
• Protein ID: WP_003409778.1
• Coordinates: 2191171..2191500 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: FPJ98_RS10035
• Protein ID: WP_003409780.1
• Coordinates: 2191497..2191874 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ98_RS10010	2187554..2188624	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
FPJ98_RS10015	2188621..2189136	+	516	WP_003409718.1	flavin reductase family protein	-
FPJ98_RS10020	2189133..2190194	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
FPJ98_RS10025	2190191..2190961	+	771	WP_003409775.1	SDR family oxidoreductase	-
FPJ98_RS10030	2191171..2191500	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FPJ98_RS10035	2191497..2191874	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
FPJ98_RS10040	2191871..2192461	-	591	WP_003409784.1	SEC-C domain-containing protein	-
FPJ98_RS10045	2192516..2193880	+	1365	WP_031648120.1	HNH endonuclease	-
FPJ98_RS10050	2194035..2194487	-	453	WP_031659767.1	hypothetical protein	-
FPJ98_RS10055	2194551..2194952	+	402	WP_003409869.1	hypothetical protein	-
FPJ98_RS10060	2194945..2195127	-	183	WP_003409870.1	hypothetical protein	-
FPJ98_RS10065	2195241..2195591	-	351	WP_003409871.1	hypothetical protein	-
FPJ98_RS10070	2195602..2196504	-	903	WP_003409874.1	hypothetical protein	-
FPJ98_RS10075	2196525..2196716	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T130669 WP_003409778.1 NZ_CP041811:c2191500-2191171 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T130669 NZ_CP041811:c2191500-2191171 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT130669 WP_003409780.1 NZ_CP041811:c2191874-2191497 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT130669 NZ_CP041811:c2191874-2191497 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8