Detailed information of TA system TA130504

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3054958..3055637
• Replicon: chromosome
• Accession: NZ_CP041808
• Organism: Mycobacterium tuberculosis strain SEA09167P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: FPK01_RS14365
• Protein ID: WP_003414059.1
• Coordinates: 3054958..3055374 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: FPK01_RS14370
• Protein ID: WP_003414061.1
• Coordinates: 3055371..3055637 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK01_RS14345	3051010..3051912	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
FPK01_RS14350	3051981..3052733	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
FPK01_RS14355	3052977..3053252	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
FPK01_RS14360	3053249..3054871	-	1623	WP_003906897.1	type I restriction-modification system subunit M	-
FPK01_RS14365	3054958..3055374	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
FPK01_RS14370	3055371..3055637	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPK01_RS14375	3055663..3056058	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
FPK01_RS14380	3056055..3056324	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
FPK01_RS14385	3056334..3057428	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
FPK01_RS14390	3057425..3057844	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
FPK01_RS14395	3057918..3058397	-	480	WP_003414073.1	dihydrofolate reductase	-
FPK01_RS14400	3058468..3059268	-	801	WP_009939104.1	thymidylate synthase	-
FPK01_RS14405	3059424..3060161	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T130504 WP_003414059.1 NZ_CP041808:c3055374-3054958 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T130504 NZ_CP041808:c3055374-3054958 [Mycobacterium tuberculosis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT130504 WP_003414061.1 NZ_CP041808:c3055637-3055371 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT130504 NZ_CP041808:c3055637-3055371 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64