Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2854649..2855280 Replicon chromosome
Accession NZ_CP041808
Organism Mycobacterium tuberculosis strain SEA09167P6C4

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag FPK01_RS13270 Protein ID WP_003413174.1
Coordinates 2854903..2855280 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag FPK01_RS13265 Protein ID WP_003413167.1
Coordinates 2854649..2854906 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPK01_RS13235 2850563..2850877 + 315 WP_009937839.1 hypothetical protein -
FPK01_RS13240 2851173..2852384 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
FPK01_RS13245 2852511..2853170 + 660 WP_003900846.1 LppA family lipoprotein -
FPK01_RS13250 2853167..2853829 + 663 WP_003900848.1 hypothetical protein -
FPK01_RS13255 2853826..2854104 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
FPK01_RS13260 2854197..2854610 + 414 WP_003413164.1 PIN domain nuclease -
FPK01_RS13265 2854649..2854906 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein Antitoxin
FPK01_RS13270 2854903..2855280 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
FPK01_RS13275 2855296..2855670 - 375 WP_003413177.1 hypothetical protein -
FPK01_RS13280 2855770..2856165 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
FPK01_RS13285 2856162..2856407 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
FPK01_RS13290 2856818..2857237 - 420 WP_003413190.1 A24 family peptidase -
FPK01_RS13295 2857249..2858058 - 810 WP_003413193.1 shikimate dehydrogenase -
FPK01_RS13300 2858055..2859308 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
FPK01_RS13305 2859301..2859813 - 513 WP_031655665.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T130500 WP_003413174.1 NZ_CP041808:2854903-2855280 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T130500 NZ_CP041808:2854903-2855280 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT130500 WP_003413167.1 NZ_CP041808:2854649-2854906 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT130500 NZ_CP041808:2854649-2854906 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCGGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References