Detailed information of TA system TA130477

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1246403..1246971
• Replicon: chromosome
• Accession: NZ_CP041808
• Organism: Mycobacterium tuberculosis strain SEA09167P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: FPK01_RS05910
• Protein ID: WP_003405865.1
• Coordinates: 1246597..1246971 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: FPK01_RS05905
• Protein ID: WP_003405863.1
• Coordinates: 1246403..1246600 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK01_RS05885	1242444..1243082	-	639	WP_003898730.1	lipid droplet-associated protein	-
FPK01_RS05890	1243172..1244179	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
FPK01_RS05895	1244196..1245179	-	984	WP_003898731.1	hypothetical protein	-
FPK01_RS05900	1245242..1246315	+	1074	WP_031655630.1	redox-regulated ATPase YchF	-
FPK01_RS05905	1246403..1246600	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPK01_RS05910	1246597..1246971	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPK01_RS05915	1247174..1247872	+	699	WP_003898733.1	hypothetical protein	-
FPK01_RS05920	1247990..1248175	+	186	WP_003901093.1	hypothetical protein	-
FPK01_RS05925	1248102..1248377	-	276	WP_003405867.1	hypothetical protein	-
FPK01_RS05930	1248620..1248943	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
FPK01_RS05935	1248958..1249668	-	711	WP_003911399.1	hypothetical protein	-
FPK01_RS05940	1249690..1249899	+	210	WP_003911400.1	hypothetical protein	-
FPK01_RS05945	1249851..1250558	-	708	Protein_1171	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T130477 WP_003405865.1 NZ_CP041808:1246597-1246971 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T130477 NZ_CP041808:1246597-1246971 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT130477 WP_003405863.1 NZ_CP041808:1246403-1246600 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT130477 NZ_CP041808:1246403-1246600 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07