Detailed information of TA system TA130444

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3060755..3061442
• Replicon: chromosome
• Accession: NZ_CP041807
• Organism: Mycobacterium tuberculosis strain SEA11020038P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: FPK02_RS14375
• Protein ID: WP_003414064.1
• Coordinates: 3061047..3061442 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: FPK02_RS14370
• Protein ID: WP_003414061.1
• Coordinates: 3060755..3061021 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK02_RS14345	3056394..3057296	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
FPK02_RS14350	3057365..3058117	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
FPK02_RS14355	3058361..3058636	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
FPK02_RS14360	3058633..3060255	-	1623	WP_003906897.1	type I restriction-modification system subunit M	-
FPK02_RS14365	3060342..3060758	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	-
FPK02_RS14370	3060755..3061021	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPK02_RS14375	3061047..3061442	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPK02_RS14380	3061439..3061708	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
FPK02_RS14385	3061718..3062812	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
FPK02_RS14390	3062809..3063228	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
FPK02_RS14395	3063302..3063781	-	480	WP_003414073.1	dihydrofolate reductase	-
FPK02_RS14400	3063852..3064652	-	801	WP_003911953.1	thymidylate synthase	-
FPK02_RS14405	3064808..3065545	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T130444 WP_003414064.1 NZ_CP041807:c3061442-3061047 [Mycobacterium tuberculosis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T130444 NZ_CP041807:c3061442-3061047 [Mycobacterium tuberculosis]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT130444 WP_003414061.1 NZ_CP041807:c3061021-3060755 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT130444 NZ_CP041807:c3061021-3060755 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64