Detailed information of TA system TA130371

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2217480..2218066
• Replicon: chromosome
• Accession: NZ_CP041806
• Organism: Mycobacterium tuberculosis strain SEA11020057P6C4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FPK03_RS10310
• Protein ID: WP_003912702.1
• Coordinates: 2217480..2217824 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL58
• Locus tag: FPK03_RS10315
• Protein ID: WP_003410014.1
• Coordinates: 2217818..2218066 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK03_RS10275	2213186..2213785	+	600	WP_003409989.1	L-lysine exporter	-
FPK03_RS10280	2214201..2214629	+	429	WP_003409992.1	cellulose-binding protein	-
FPK03_RS10285	2214855..2215394	+	540	WP_003900446.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37)	-
FPK03_RS10290	2215914..2216474	-	561	WP_003410001.1	RES family NAD+ phosphorylase	-
FPK03_RS10295	2216471..2216812	-	342	WP_003410003.1	DUF2384 domain-containing protein	-
FPK03_RS10300	2216898..2217155	+	258	WP_003410006.1	hypothetical protein	-
FPK03_RS10305	2217056..2217391	-	336	WP_003410009.1	dehydrogenase	-
FPK03_RS10310	2217480..2217824	-	345	WP_003912702.1	type II toxin-antitoxin system toxin endoribonuclease MazF6	Toxin
FPK03_RS10315	2217818..2218066	-	249	WP_003410014.1	ribbon-helix-helix protein, CopG family	Antitoxin
FPK03_RS10320	2218166..2220481	-	2316	WP_003899120.1	cation transporter ATPase CptG	-
FPK03_RS10325	2220478..2220750	-	273	WP_003410017.1	DUF1490 family protein	-
FPK03_RS10330	2220803..2221159	-	357	WP_003410018.1	Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR	-
FPK03_RS10335	2221316..2222083	+	768	WP_003410019.1	hemerythrin domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-38)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12260.15 Da
• Isoelectric Point: 9.8887

>T130371 WP_003912702.1 NZ_CP041806:c2217824-2217480 [Mycobacterium tuberculosis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPSNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Nucleotide

• Length: 345 bp

>T130371 NZ_CP041806:c2217824-2217480 [Mycobacterium tuberculosis]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCAGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9290.34 Da
• Isoelectric Point: 4.3757

>AT130371 WP_003410014.1 NZ_CP041806:c2218066-2217818 [Mycobacterium tuberculosis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Nucleotide

• Length: 249 bp

>AT130371 NZ_CP041806:c2218066-2217818 [Mycobacterium tuberculosis]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U4FAW9