Detailed information of TA system TA130366

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2177818..2178521
• Replicon: chromosome
• Accession: NZ_CP041806
• Organism: Mycobacterium tuberculosis strain SEA11020057P6C4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: FPK03_RS10035
• Protein ID: WP_003409778.1
• Coordinates: 2177818..2178147 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: FPK03_RS10040
• Protein ID: WP_003409780.1
• Coordinates: 2178144..2178521 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK03_RS10015	2174201..2175271	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
FPK03_RS10020	2175268..2175783	+	516	WP_003409718.1	flavin reductase family protein	-
FPK03_RS10025	2175780..2176841	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
FPK03_RS10030	2176838..2177608	+	771	WP_003409775.1	SDR family oxidoreductase	-
FPK03_RS10035	2177818..2178147	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FPK03_RS10040	2178144..2178521	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
FPK03_RS10045	2178518..2179108	-	591	WP_003409784.1	SEC-C domain-containing protein	-
FPK03_RS10050	2179163..2180527	+	1365	WP_031656246.1	HNH endonuclease	-
FPK03_RS10055	2180682..2181134	-	453	WP_003899095.1	lipoprotein	-
FPK03_RS10060	2181198..2181599	+	402	WP_003409869.1	hypothetical protein	-
FPK03_RS10065	2181592..2181774	-	183	WP_003409870.1	hypothetical protein	-
FPK03_RS10070	2181888..2182238	-	351	WP_003899096.1	hypothetical protein	-
FPK03_RS10075	2182249..2183151	-	903	WP_003899097.1	hypothetical protein	-
FPK03_RS10080	2183172..2183363	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T130366 WP_003409778.1 NZ_CP041806:c2178147-2177818 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T130366 NZ_CP041806:c2178147-2177818 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT130366 WP_003409780.1 NZ_CP041806:c2178521-2178144 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT130366 NZ_CP041806:c2178521-2178144 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8