Detailed information of TA system TA130348

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 756307..756940
• Replicon: chromosome
• Accession: NZ_CP041806
• Organism: Mycobacterium tuberculosis strain SEA11020057P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FPK03_RS03460
• Protein ID: WP_003912919.1
• Coordinates: 756307..756690 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ56
• Locus tag: FPK03_RS03465
• Protein ID: WP_003403368.1
• Coordinates: 756785..756940 (-)
• Length: 52 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK03_RS03435	751599..752135	+	537	WP_003403341.1	50S ribosomal protein L10	-
FPK03_RS03440	752172..752564	+	393	WP_003403353.1	50S ribosomal protein L7/L12	-
FPK03_RS03445	752557..753252	-	696	WP_003403355.1	TetR/AcrR family transcriptional regulator	-
FPK03_RS03450	753323..754828	+	1506	WP_003403360.1	carotenoid cleavage oxygenase	-
FPK03_RS03455	754909..755919	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
FPK03_RS03460	756307..756690	-	384	WP_003912919.1	ribonuclease VapC6	Toxin
FPK03_RS03465	756785..756940	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPK03_RS03470	757016..757732	-	717	WP_193018901.1	CPBP family intramembrane metalloprotease	-
FPK03_RS03475	758008..758316	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
FPK03_RS03480	758303..758548	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	-
FPK03_RS03485	758658..759095	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	-
FPK03_RS03490	759092..759346	-	255	WP_003911263.1	antitoxin VapB7	-
FPK03_RS03495	759460..761823	+	2364	WP_003898547.1	arylsulfatase AtsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-44)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13989.76 Da
• Isoelectric Point: 6.0803

>T130348 WP_003912919.1 NZ_CP041806:c756690-756307 [Mycobacterium tuberculosis]
MAAATTTGTHRGLELRAAQRAVGSCEPQRAEFCRSARNADEFDQMSRMFGDVYPDVPVPKSVWRWIDSAQHRLARAGAVG
ALSVVDLLICDTATARGLVVLHDDADYELAERHLPDIRVRRVVSADD

Nucleotide

• Length: 384 bp

>T130348 NZ_CP041806:c756690-756307 [Mycobacterium tuberculosis]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATCAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTGGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGATCTGCGACACTGCGACGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin

Protein

• Length: 52 a.a.
• Molecular weight: 5872.72 Da
• Isoelectric Point: 5.7734

>AT130348 WP_003403368.1 NZ_CP041806:c756940-756785 [Mycobacterium tuberculosis]
VSVTQIDLDDEALADVMRIAAVHTKKEAVNLAMRDYVERFRRIEALARSRE

Nucleotide

• Length: 156 bp

>AT130348 NZ_CP041806:c756940-756785 [Mycobacterium tuberculosis]
GTGTCGGTGACCCAGATCGACCTCGACGACGAAGCGCTGGCCGATGTGATGCGCATCGCCGCCGTGCACACAAAGAAGGA
AGCCGTGAACCTCGCGATGCGCGATTACGTCGAACGGTTTCGGCGCATCGAGGCCCTGGCCCGCTCGCGCGAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U4BSF8