Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 3132881..3133485 Replicon chromosome
Accession NZ_CP041805
Organism Mycobacterium tuberculosis strain SEA11020068P6C4

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag FPK04_RS14760 Protein ID WP_003414492.1
Coordinates 3132881..3133273 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A829CBY8
Locus tag FPK04_RS14765 Protein ID WP_003414495.1
Coordinates 3133270..3133485 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPK04_RS14730 3128031..3128819 - 789 WP_003917092.1 CRISPR-associated endoribonuclease Cas6 -
FPK04_RS14735 3129153..3129698 - 546 WP_003913758.1 DUF1802 family protein -
FPK04_RS14740 3129970..3130854 - 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
FPK04_RS14745 3130857..3131744 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
FPK04_RS14750 3132049..3132594 - 546 WP_003899500.1 DUF1802 family protein -
FPK04_RS14755 3132591..3132860 - 270 WP_003912856.1 DUF2277 family protein -
FPK04_RS14760 3132881..3133273 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
FPK04_RS14765 3133270..3133485 - 216 WP_003414495.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
FPK04_RS14770 3133532..3134281 + 750 WP_003414497.1 enoyl-CoA hydratase -
FPK04_RS14775 3134360..3135442 - 1083 WP_003414499.1 ABC transporter ATP-binding protein -
FPK04_RS14780 3135435..3136745 - 1311 WP_003414501.1 ABC transporter substrate-binding protein -
FPK04_RS14785 3136748..3137575 - 828 WP_003414504.1 carbohydrate ABC transporter permease -
FPK04_RS14790 3137572..3138483 - 912 WP_003414505.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T130327 WP_003414492.1 NZ_CP041805:c3133273-3132881 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T130327 NZ_CP041805:c3133273-3132881 [Mycobacterium tuberculosis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTTTGGTAG

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7511.55 Da        Isoelectric Point: 4.9732

>AT130327 WP_003414495.1 NZ_CP041805:c3133485-3133270 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Download         Length: 216 bp

>AT130327 NZ_CP041805:c3133485-3133270 [Mycobacterium tuberculosis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CBY8

References