Detailed information of TA system TA130323

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3066692..3067371
• Replicon: chromosome
• Accession: NZ_CP041805
• Organism: Mycobacterium tuberculosis strain SEA11020068P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: FPK04_RS14395
• Protein ID: WP_003414059.1
• Coordinates: 3066692..3067108 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: FPK04_RS14400
• Protein ID: WP_003414061.1
• Coordinates: 3067105..3067371 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK04_RS14375	3062744..3063646	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
FPK04_RS14380	3063715..3064467	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
FPK04_RS14385	3064711..3064986	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
FPK04_RS14390	3064983..3066605	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
FPK04_RS14395	3066692..3067108	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
FPK04_RS14400	3067105..3067371	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPK04_RS14405	3067397..3067792	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
FPK04_RS14410	3067789..3068058	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
FPK04_RS14415	3068068..3069162	-	1095	WP_003900565.1	restriction endonuclease subunit S	-
FPK04_RS14420	3069159..3069578	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
FPK04_RS14425	3069652..3070131	-	480	WP_003414073.1	dihydrofolate reductase	-
FPK04_RS14430	3070202..3071002	-	801	WP_003911953.1	thymidylate synthase	-
FPK04_RS14435	3071158..3071895	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T130323 WP_003414059.1 NZ_CP041805:c3067108-3066692 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T130323 NZ_CP041805:c3067108-3066692 [Mycobacterium tuberculosis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT130323 WP_003414061.1 NZ_CP041805:c3067371-3067105 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT130323 NZ_CP041805:c3067371-3067105 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64