Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2867507..2868144 Replicon chromosome
Accession NZ_CP041805
Organism Mycobacterium tuberculosis strain SEA11020068P6C4

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag FPK04_RS13305 Protein ID WP_003413180.1
Coordinates 2867507..2867902 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag FPK04_RS13310 Protein ID WP_003413183.1
Coordinates 2867899..2868144 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPK04_RS13265 2863592..2864251 + 660 WP_003900846.1 LppA family lipoprotein -
FPK04_RS13270 2864248..2864907 + 660 WP_003900847.1 hypothetical protein -
FPK04_RS13275 2864904..2865566 + 663 WP_003900848.1 hypothetical protein -
FPK04_RS13280 2865563..2865841 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
FPK04_RS13285 2865934..2866347 + 414 WP_003413164.1 PIN domain nuclease -
FPK04_RS13290 2866386..2866643 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
FPK04_RS13295 2866640..2867017 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
FPK04_RS13300 2867033..2867407 - 375 WP_003413177.1 hypothetical protein -
FPK04_RS13305 2867507..2867902 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
FPK04_RS13310 2867899..2868144 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
FPK04_RS13315 2868555..2868974 - 420 WP_003413190.1 A24 family peptidase -
FPK04_RS13320 2868986..2869795 - 810 WP_003413193.1 shikimate dehydrogenase -
FPK04_RS13325 2869792..2871045 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
FPK04_RS13330 2871038..2871550 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T130320 WP_003413180.1 NZ_CP041805:c2867902-2867507 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T130320 NZ_CP041805:c2867902-2867507 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT130320 WP_003413183.1 NZ_CP041805:c2868144-2867899 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT130320 NZ_CP041805:c2868144-2867899 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References