Detailed information of TA system TA130235

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1239311..1239879
• Replicon: chromosome
• Accession: NZ_CP041804
• Organism: Mycobacterium tuberculosis strain SEA11020092P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: FPK05_RS05870
• Protein ID: WP_003405865.1
• Coordinates: 1239505..1239879 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: FPK05_RS05865
• Protein ID: WP_003405863.1
• Coordinates: 1239311..1239508 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK05_RS05845	1235352..1235990	-	639	WP_003898730.1	lipid droplet-associated protein	-
FPK05_RS05850	1236080..1237087	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
FPK05_RS05855	1237104..1238087	-	984	WP_003898731.1	hypothetical protein	-
FPK05_RS05860	1238150..1239223	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
FPK05_RS05865	1239311..1239508	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPK05_RS05870	1239505..1239879	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPK05_RS05875	1240082..1240780	+	699	WP_003898733.1	hypothetical protein	-
FPK05_RS05880	1240898..1241083	+	186	WP_003901093.1	hypothetical protein	-
FPK05_RS05885	1241010..1241285	-	276	WP_003405867.1	hypothetical protein	-
FPK05_RS05890	1241528..1241851	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
FPK05_RS05895	1241866..1242576	-	711	WP_003911399.1	hypothetical protein	-
FPK05_RS05900	1242598..1242807	+	210	WP_003911400.1	hypothetical protein	-
FPK05_RS05905	1242759..1243466	-	708	Protein_1163	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T130235 WP_003405865.1 NZ_CP041804:1239505-1239879 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T130235 NZ_CP041804:1239505-1239879 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT130235 WP_003405863.1 NZ_CP041804:1239311-1239508 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT130235 NZ_CP041804:1239311-1239508 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07