Detailed information of TA system TA130201

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3065470..3066149
• Replicon: chromosome
• Accession: NZ_CP041803
• Organism: Mycobacterium tuberculosis strain SEA11278P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: FPK06_RS14310
• Protein ID: WP_003414059.1
• Coordinates: 3065470..3065886 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: FPK06_RS14315
• Protein ID: WP_003414061.1
• Coordinates: 3065883..3066149 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK06_RS14290	3061522..3062424	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
FPK06_RS14295	3062493..3063245	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
FPK06_RS14300	3063489..3063764	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
FPK06_RS14305	3063761..3065383	-	1623	WP_050069575.1	type I restriction-modification system subunit M	-
FPK06_RS14310	3065470..3065886	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
FPK06_RS14315	3065883..3066149	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPK06_RS14320	3066175..3066570	-	396	WP_193019633.1	type II toxin-antitoxin system VapC family toxin	-
FPK06_RS14325	3066567..3066836	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
FPK06_RS14330	3066846..3067940	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
FPK06_RS14335	3067937..3068356	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
FPK06_RS14340	3068430..3068909	-	480	WP_003414073.1	dihydrofolate reductase	-
FPK06_RS14345	3068980..3069780	-	801	WP_003911953.1	thymidylate synthase	-
FPK06_RS14350	3069936..3070673	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T130201 WP_003414059.1 NZ_CP041803:c3065886-3065470 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T130201 NZ_CP041803:c3065886-3065470 [Mycobacterium tuberculosis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT130201 WP_003414061.1 NZ_CP041803:c3066149-3065883 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT130201 NZ_CP041803:c3066149-3065883 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64