Detailed information of TA system TA130168

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-mazE/PIN(toxin)
• Location: 758554..759346
• Replicon: chromosome
• Accession: NZ_CP041803
• Organism: Mycobacterium tuberculosis strain SEA11278P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WFB2
• Locus tag: FPK06_RS03480
• Protein ID: WP_003403386.1
• Coordinates: 758909..759346 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TQE0
• Locus tag: FPK06_RS03475
• Protein ID: WP_003403381.1
• Coordinates: 758554..758799 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK06_RS03445	753573..755079	+	1507	Protein_680	carotenoid cleavage oxygenase	-
FPK06_RS03450	755160..756170	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
FPK06_RS03455	756558..756941	-	384	WP_003403365.1	ribonuclease VapC6	-
FPK06_RS03460	757036..757191	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	-
FPK06_RS03465	757267..757983	-	717	WP_003403371.1	CPBP family intramembrane metalloprotease	-
FPK06_RS03470	758259..758567	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
FPK06_RS03475	758554..758799	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	Antitoxin
FPK06_RS03480	758909..759346	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPK06_RS03485	759343..759597	-	255	WP_003911263.1	antitoxin VapB7	-
FPK06_RS03490	759711..762074	+	2364	WP_003403397.1	arylsulfatase AtsD	-
FPK06_RS03495	762137..762463	+	327	WP_003403401.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FPK06_RS03500	762375..762713	+	339	WP_003403405.1	PIN domain-containing protein	-
FPK06_RS03505	762710..762883	+	174	WP_003898549.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-126)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15245.39 Da
• Isoelectric Point: 5.1865

>T130168 WP_003403386.1 NZ_CP041803:c759346-758909 [Mycobacterium tuberculosis]
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR

Nucleotide

• Length: 438 bp

>T130168 NZ_CP041803:c759346-758909 [Mycobacterium tuberculosis]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9169.01 Da
• Isoelectric Point: 4.2070

>AT130168 WP_003403381.1 NZ_CP041803:c758799-758554 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Nucleotide

• Length: 246 bp

>AT130168 NZ_CP041803:c758799-758554 [Mycobacterium tuberculosis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSW5

Antitoxin

• AlphaFold DB: G0TQE0