Detailed information of TA system TA130165

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-PHD
• Location: 721056..721720
• Replicon: chromosome
• Accession: NZ_CP041803
• Organism: Mycobacterium tuberculosis strain SEA11278P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P96917
• Locus tag: FPK06_RS03285
• Protein ID: WP_003403246.1
• Coordinates: 721313..721720 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: FPK06_RS03280
• Protein ID: WP_050069288.1
• Coordinates: 721056..721316 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK06_RS03255	717233..718390	+	1158	WP_003903091.1	hypothetical protein	-
FPK06_RS03260	718401..719348	+	948	WP_050069287.1	DUF732 domain-containing protein	-
FPK06_RS03265	719441..719695	+	255	WP_003403235.1	type II toxin-antitoxin system antitoxin VapB30	-
FPK06_RS03270	719695..720090	+	396	WP_003403236.1	type II toxin-antitoxin system toxin ribonuclease C30	-
FPK06_RS03275	720184..720924	-	741	WP_003403239.1	TVP38/TMEM64 family protein	-
FPK06_RS03280	721056..721316	+	261	WP_050069288.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FPK06_RS03285	721313..721720	+	408	WP_003403246.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPK06_RS03290	721792..722943	-	1152	WP_050069289.1	FIST C-terminal domain-containing protein	-
FPK06_RS03295	723036..724763	-	1728	WP_003900979.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-125)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-57)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14376.37 Da
• Isoelectric Point: 4.3568

>T130165 WP_003403246.1 NZ_CP041803:721313-721720 [Mycobacterium tuberculosis]
VSTTPAAGVLDTSVFIATESGRQLDEALIPDRVATTVVTLAELRVGVLAAATTDIRAQRLATLESVADMETLPVDDDAAR
MWARLRIHLAESGRRVRINDLWIAAVAASRALPVITQDDDFAALDGAASVEIIRV

Nucleotide

• Length: 408 bp

>T130165 NZ_CP041803:721313-721720 [Mycobacterium tuberculosis]
GTGAGCACGACGCCGGCCGCCGGAGTGCTCGACACGTCGGTGTTCATCGCGACCGAAAGCGGCCGGCAACTCGACGAGGC
GCTGATCCCCGACCGGGTCGCCACCACCGTCGTCACCCTCGCCGAACTGCGCGTCGGCGTGCTGGCCGCGGCGACGACCG
ACATCCGGGCTCAACGCCTGGCGACCCTGGAATCCGTTGCCGATATGGAAACGTTGCCCGTCGACGACGATGCCGCCCGA
ATGTGGGCCCGATTGCGGATCCATCTTGCCGAGTCCGGTCGCCGGGTGCGGATCAACGACCTGTGGATCGCGGCCGTCGC
GGCATCGCGAGCGCTGCCGGTCATCACCCAGGACGACGACTTCGCCGCCCTCGACGGTGCGGCCAGTGTGGAGATCATTC
GGGTCTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9493.83 Da
• Isoelectric Point: 11.7137

>AT130165 WP_050069288.1 NZ_CP041803:721056-721316 [Mycobacterium tuberculosis]
MSEVASRELRNDTAGVLRRVRAGEDVTITVSGRPVAVLTPVRPRRRRWLSKTEFLSRLRGAQADPGLRDDLAVLAGDTTE
DLGPIR

Nucleotide

• Length: 261 bp

>AT130165 NZ_CP041803:721056-721316 [Mycobacterium tuberculosis]
ATGTCTGAGGTGGCCTCGCGTGAGCTGCGTAACGATACGGCCGGCGTGCTGCGCCGCGTGCGGGCAGGGGAGGACGTCAC
CATCACCGTCAGCGGCCGTCCGGTCGCGGTGCTTACCCCGGTTCGTCCGCGGCGCCGGCGTTGGCTGAGCAAAACGGAGT
TCCTGTCGCGGTTGCGCGGCGCTCAAGCCGATCCCGGGCTCCGTGACGACCTCGCGGTCCTTGCCGGCGACACGACCGAG
GATCTCGGGCCGATCCGGTGA

Structures

Toxin

• PDB: 3DBO