Detailed information of TA system TA130158

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 643355..644031
• Replicon: chromosome
• Accession: NZ_CP041803
• Organism: Mycobacterium tuberculosis strain SEA11278P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TPQ9
• Locus tag: FPK06_RS02880
• Protein ID: WP_003898500.1
• Coordinates: 643355..643768 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TPR0
• Locus tag: FPK06_RS02885
• Protein ID: WP_003402915.1
• Coordinates: 643765..644031 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK06_RS02850	638700..639002	-	303	WP_003402896.1	DUF3349 domain-containing protein	-
FPK06_RS02855	639062..639340	-	279	WP_050069374.1	hypothetical protein	-
FPK06_RS02860	639337..640590	-	1254	WP_003402904.1	inorganic phosphate transporter family protein	-
FPK06_RS02865	640677..641096	-	420	WP_050069373.1	VOC family protein	-
FPK06_RS02870	641159..642043	-	885	WP_003402909.1	SDR family oxidoreductase	-
FPK06_RS02875	642139..643041	-	903	WP_003915439.1	1,4-dihydroxy-2-naphthoyl-CoA synthase	-
FPK06_RS02880	643355..643768	-	414	WP_003898500.1	PIN domain-containing protein	Toxin
FPK06_RS02885	643765..644031	-	267	WP_003402915.1	antitoxin VapB3	Antitoxin
FPK06_RS02890	644223..645939	-	1717	Protein_573	AMP-binding protein	-
FPK06_RS02895	646017..647621	+	1605	WP_003402920.1	amidohydrolase	-
FPK06_RS02900	647618..648598	+	981	WP_003402923.1	o-succinylbenzoate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (13-129)

Antitoxin

• Post-segregation antitoxin CcdA (19-61)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14667.78 Da
• Isoelectric Point: 7.3603

>T130158 WP_003898500.1 NZ_CP041803:c643768-643355 [Mycobacterium tuberculosis]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Nucleotide

• Length: 414 bp

>T130158 NZ_CP041803:c643768-643355 [Mycobacterium tuberculosis]
GTGAGAGCATCGCCCACTTCGCCGCCGGAGCAGGTGGTCGTCGACGCGAGTGCCATGGTGGATCTACTGGCTCGCACTAG
CGATCGGTGCTCTGCGGTGCGCGCGCGGCTGGCTCGGACCGCGATGCACGCGCCGGCGCACTTCGATGCAGAGGTGTTGT
CGGCGCTGGGGCGCATGCAGCGCGCCGGCGCACTCACCGTTGCCTATGTCGATGCGGCACTGGAGGAGTTGCGACAGGTG
CCGGTGACTCGACACGGTCTTTCGTCGCTGCTTGCTGGAGCGTGGTCGCGCCGCGACACCCTCCGCCTGACCGATGCCCT
CTACGTCGAGCTGGCCGAAACGGCAGGTCTGGTGTTGTTGACCACCGACGAAAGATTGGCACGCGCCTGGCCCTCGGCTC
ACGCCATCGGCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9543.79 Da
• Isoelectric Point: 4.5994

>AT130158 WP_003402915.1 NZ_CP041803:c644031-643765 [Mycobacterium tuberculosis]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Nucleotide

• Length: 267 bp

>AT130158 NZ_CP041803:c644031-643765 [Mycobacterium tuberculosis]
TTGTTGAGCCGGCGCACGAAAACGATCGTTGTGTGTACATTGGTGTGTATGGCTCGGTTGAACGTGTATGTGCCCGACGA
ATTGGCGGAGCGCGCCAGGGCGCGGGGCTTGAACGTCTCGGCGCTGACTCAGGCCGCGATCAGTGCCGAGTTGGAGAACT
CCGCAACCGATGCGTGGCTTGAGGGGTTGGAACCCAGAAGCACCGGCGCTCGGCATGATGACGTGCTGGGTGCGATCGAT
GCCGCTCGCGATGAGTTCGAAGCGTGA

Structures

Toxin

• AlphaFold DB: G0TPQ9

Antitoxin

• AlphaFold DB: G0TPR0