Detailed information of TA system TA130145

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3133972..3134576
• Replicon: chromosome
• Accession: NZ_CP041802
• Organism: Mycobacterium tuberculosis strain SEA12126P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: FPK07_RS14710
• Protein ID: WP_003414492.1
• Coordinates: 3133972..3134364 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A829CBY8
• Locus tag: FPK07_RS14715
• Protein ID: WP_003414495.1
• Coordinates: 3134361..3134576 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK07_RS14680	3129122..3129910	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
FPK07_RS14685	3130244..3130789	-	546	WP_003904931.1	DUF1802 family protein	-
FPK07_RS14690	3131061..3131945	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
FPK07_RS14695	3131948..3132835	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
FPK07_RS14700	3133140..3133685	-	546	WP_003904931.1	DUF1802 family protein	-
FPK07_RS14705	3133682..3133951	-	270	WP_003912856.1	DUF2277 family protein	-
FPK07_RS14710	3133972..3134364	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPK07_RS14715	3134361..3134576	-	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FPK07_RS14720	3134623..3135372	+	750	WP_003414497.1	enoyl-CoA hydratase	-
FPK07_RS14725	3135451..3136533	-	1083	Protein_2906	ABC transporter ATP-binding protein	-
FPK07_RS14730	3136526..3137836	-	1311	WP_003414501.1	ABC transporter substrate-binding protein	-
FPK07_RS14735	3137839..3138666	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
FPK07_RS14740	3138663..3139574	-	912	WP_003414505.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T130145 WP_003414492.1 NZ_CP041802:c3134364-3133972 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T130145 NZ_CP041802:c3134364-3133972 [Mycobacterium tuberculosis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7511.55 Da
• Isoelectric Point: 4.9732

>AT130145 WP_003414495.1 NZ_CP041802:c3134576-3134361 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT130145 NZ_CP041802:c3134576-3134361 [Mycobacterium tuberculosis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8

Antitoxin

• AlphaFold DB: A0A829CBY8