Detailed information of TA system TA130032

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-PHD
• Location: 3821005..3821672
• Replicon: chromosome
• Accession: NZ_CP041800
• Organism: Mycobacterium tuberculosis strain SEA12334P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: O50411
• Locus tag: FPK09_RS17730
• Protein ID: WP_003417916.1
• Coordinates: 3821005..3821397 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: FPK09_RS17735
• Protein ID: WP_031674613.1
• Coordinates: 3821397..3821672 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK09_RS17715	3816378..3818216	-	1839	WP_019830554.1	1-deoxy-D-xylulose-5-phosphate synthase	-
FPK09_RS17720	3818213..3819202	-	990	WP_003417912.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
FPK09_RS17725	3819202..3820254	-	1053	WP_003900678.1	polyprenyl synthetase family protein	-
FPK09_RS17730	3821005..3821397	-	393	WP_003417916.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPK09_RS17735	3821397..3821672	-	276	WP_031674613.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
FPK09_RS17740	3821884..3823225	+	1342	Protein_3504	ISNCY family transposase	-
FPK09_RS17745	3823415..3825670	+	2256	WP_031674615.1	PE family protein	-
FPK09_RS17750	3825741..3826613	-	873	WP_003417929.1	3-hydroxyacyl-thioester dehydratase HtdY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3815479..3825670	10191
	-	flank	IS/Tn	-	-	3822548..3823225	677

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-53)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14316.74 Da
• Isoelectric Point: 10.7249

>T130032 WP_003417916.1 NZ_CP041800:c3821397-3821005 [Mycobacterium tuberculosis]
MAAIYLDSSAIVKLAVREPESDALRRYLRTRHPRVSSALARAEVMRALLDKGESARKAGRRALAHLDLLRVDKRVLDLAG
GLLPFELRTLDAIHLATAQRLGVDLGRLCTYDDRMRDAAKTLGMAVIAPS

Nucleotide

• Length: 393 bp

>T130032 NZ_CP041800:c3821397-3821005 [Mycobacterium tuberculosis]
ATGGCGGCCATCTATCTCGACTCGTCCGCGATCGTCAAGCTCGCGGTTCGCGAGCCAGAGTCGGACGCGCTGCGCCGCTA
CCTTCGCACCCGCCACCCGCGAGTGTCGAGTGCCCTTGCGCGCGCGGAGGTCATGCGGGCCCTGCTTGACAAGGGTGAAT
CAGCGCGGAAAGCCGGCCGCCGGGCGCTGGCTCATCTCGATCTGCTGCGCGTCGACAAGCGCGTCCTCGATCTCGCTGGT
GGGCTGCTGCCATTCGAGCTGCGCACGCTCGATGCCATCCACTTGGCGACGGCGCAGCGGCTCGGCGTCGATCTGGGCCG
GTTGTGCACCTACGACGACCGGATGCGCGACGCCGCCAAGACGCTCGGCATGGCCGTGATCGCCCCGTCGTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 9956.31 Da
• Isoelectric Point: 4.5332

>AT130032 WP_031674613.1 NZ_CP041800:c3821672-3821397 [Mycobacterium tuberculosis]
MTSVGVRALRQRASELLRRVEAGETIEITDRGRPVALLSPLPQGGPYEQLLASGEIERATLDVVDLSEPLDLDAGVELPS
VTLARLREHER

Nucleotide

• Length: 276 bp

>AT130032 NZ_CP041800:c3821672-3821397 [Mycobacterium tuberculosis]
ATGACCTCGGTGGGGGTGCGTGCGCTGCGGCAGCGGGCCAGCGAGCTGCTGCGCCGAGTCGAGGCCGGCGAGACGATCGA
GATCACCGACCGCGGCCGGCCCGTCGCCCTGCTCTCGCCGCTGCCGCAGGGCGGTCCATACGAGCAGCTGCTGGCAAGCG
GCGAGATCGAGCGTGCCACGCTCGACGTCGTTGATCTGTCCGAACCGCTCGACCTCGACGCGGGCGTCGAGCTGCCGTCG
GTGACGCTTGCGCGCCTGCGTGAGCACGAGCGTTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BXS8