Detailed information of TA system TA130028

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 3198581..3199129
• Replicon: chromosome
• Accession: NZ_CP041800
• Organism: Mycobacterium tuberculosis strain SEA12334P6C4

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TFG5
• Locus tag: FPK09_RS15065
• Protein ID: WP_003414602.1
• Coordinates: 3198866..3199129 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: O33347
• Locus tag: FPK09_RS15060
• Protein ID: WP_003414599.1
• Coordinates: 3198581..3198862 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK09_RS15035	3194204..3195061	-	858	WP_003899513.1	type I methionyl aminopeptidase	-
FPK09_RS15040	3195103..3195687	-	585	WP_003414585.1	DUF1707 domain-containing protein	-
FPK09_RS15045	3195791..3196039	+	249	WP_003913411.1	antitoxin VapB23	-
FPK09_RS15050	3196036..3196416	+	381	WP_003414592.1	type II toxin-antitoxin system VapC family toxin	-
FPK09_RS15055	3196498..3198309	-	1812	WP_003414596.1	penicillin-binding protein	-
FPK09_RS15060	3198581..3198862	+	282	WP_003414599.1	type II toxin-antitoxin system antitoxin RelF	Antitoxin
FPK09_RS15065	3198866..3199129	+	264	WP_003414602.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FPK09_RS15070	3199502..3200356	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
FPK09_RS15075	3200412..3201575	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
FPK09_RS15080	3201592..3202806	-	1215	WP_003414610.1	zinc metalloprotease Rip	-
FPK09_RS15085	3202814..3204055	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10204.82 Da
• Isoelectric Point: 11.5078

>T130028 WP_003414602.1 NZ_CP041800:3198866-3199129 [Mycobacterium tuberculosis]
VPYTVRFTTTARRDLHKLPPRILAAVVEFAFGDLSREPLRVGKPLRRELAGTFSARRGTYRLLYRIDDEHTTVVILRVDH
RADIYRR

Nucleotide

• Length: 264 bp

>T130028 NZ_CP041800:3198866-3199129 [Mycobacterium tuberculosis]
GTGCCTTACACCGTGCGGTTCACCACAACCGCGCGTCGAGACCTCCACAAGCTGCCACCGCGAATCCTCGCGGCAGTGGT
CGAATTCGCGTTCGGCGATCTGTCGCGCGAGCCCCTGCGGGTGGGCAAGCCCCTTCGGCGCGAGTTGGCCGGCACGTTCA
GCGCGCGTCGCGGAACGTACCGCCTGCTGTACCGGATTGACGACGAGCACACAACGGTAGTGATCCTGCGCGTCGATCAC
CGCGCGGACATCTACCGCCGATAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10183.40 Da
• Isoelectric Point: 4.3440

>AT130028 WP_003414599.1 NZ_CP041800:3198581-3198862 [Mycobacterium tuberculosis]
MRILPISTIKGKLNEFVDAVSSTQDQITITKNGAPAAVLVGADEWESLQETLYWLAQPGIRESIAEADADIASGRTYGED
EIRAEFGVPRRPH

Nucleotide

• Length: 282 bp

>AT130028 NZ_CP041800:3198581-3198862 [Mycobacterium tuberculosis]
ATGCGGATACTGCCGATTTCGACGATCAAGGGCAAGCTCAATGAGTTCGTCGACGCGGTCTCGTCGACACAGGACCAGAT
CACCATCACCAAGAACGGTGCACCCGCAGCCGTTCTGGTCGGCGCCGACGAGTGGGAATCGTTGCAGGAGACGCTGTACT
GGCTGGCGCAACCCGGAATCAGGGAGTCGATCGCTGAAGCCGACGCCGACATTGCCTCCGGCCGCACCTACGGCGAAGAC
GAGATCCGCGCCGAATTCGGCGTCCCGCGACGCCCCCACTGA

Structures

Toxin

• PDB: 3G5O

Antitoxin

• AlphaFold DB: A0A7U4BWP8