Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2892049..2892686 Replicon chromosome
Accession NZ_CP041800
Organism Mycobacterium tuberculosis strain SEA12334P6C4

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag FPK09_RS13420 Protein ID WP_003413180.1
Coordinates 2892049..2892444 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag FPK09_RS13425 Protein ID WP_003413183.1
Coordinates 2892441..2892686 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPK09_RS13380 2888134..2888793 + 660 WP_031674408.1 LppA family lipoprotein -
FPK09_RS13385 2888790..2889449 + 660 WP_003900847.1 hypothetical protein -
FPK09_RS13390 2889446..2890108 + 663 WP_003903936.1 hypothetical protein -
FPK09_RS13395 2890105..2890383 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
FPK09_RS13400 2890476..2890889 + 414 WP_003413164.1 PIN domain nuclease -
FPK09_RS13405 2890928..2891185 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
FPK09_RS13410 2891182..2891559 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
FPK09_RS13415 2891575..2891949 - 375 WP_003413177.1 hypothetical protein -
FPK09_RS13420 2892049..2892444 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
FPK09_RS13425 2892441..2892686 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
FPK09_RS13430 2893097..2893516 - 420 WP_003413190.1 A24 family peptidase -
FPK09_RS13435 2893528..2894337 - 810 WP_003413193.1 shikimate dehydrogenase -
FPK09_RS13440 2894334..2895587 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
FPK09_RS13445 2895580..2896092 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T130020 WP_003413180.1 NZ_CP041800:c2892444-2892049 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T130020 NZ_CP041800:c2892444-2892049 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT130020 WP_003413183.1 NZ_CP041800:c2892686-2892441 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT130020 NZ_CP041800:c2892686-2892441 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References