Detailed information of TA system TA130005

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2209725..2210428
• Replicon: chromosome
• Accession: NZ_CP041800
• Organism: Mycobacterium tuberculosis strain SEA12334P6C4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: FPK09_RS10130
• Protein ID: WP_003409778.1
• Coordinates: 2209725..2210054 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: FPK09_RS10135
• Protein ID: WP_003409780.1
• Coordinates: 2210051..2210428 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK09_RS10110	2206108..2207178	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
FPK09_RS10115	2207175..2207690	+	516	WP_003409718.1	flavin reductase family protein	-
FPK09_RS10120	2207687..2208748	+	1062	Protein_1999	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
FPK09_RS10125	2208745..2209515	+	771	WP_003409775.1	SDR family oxidoreductase	-
FPK09_RS10130	2209725..2210054	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FPK09_RS10135	2210051..2210428	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
FPK09_RS10140	2210425..2211015	-	591	WP_003409784.1	SEC-C domain-containing protein	-
FPK09_RS10145	2211070..2212434	+	1365	WP_003903691.1	HNH endonuclease	-
FPK09_RS10150	2212589..2213041	-	453	WP_003899095.1	lipoprotein	-
FPK09_RS10155	2213105..2213506	+	402	WP_003409869.1	hypothetical protein	-
FPK09_RS10160	2213499..2213681	-	183	WP_003409870.1	hypothetical protein	-
FPK09_RS10165	2213795..2214145	-	351	WP_003409871.1	hypothetical protein	-
FPK09_RS10170	2214156..2215058	-	903	WP_003409874.1	hypothetical protein	-
FPK09_RS10175	2215079..2215270	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T130005 WP_003409778.1 NZ_CP041800:c2210054-2209725 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T130005 NZ_CP041800:c2210054-2209725 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT130005 WP_003409780.1 NZ_CP041800:c2210428-2210051 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT130005 NZ_CP041800:c2210428-2210051 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8