Detailed information of TA system TA129999

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PhRel-ATphRel/-
• Location: 1547359..1548409
• Replicon: chromosome
• Accession: NZ_CP041800
• Organism: Mycobacterium tuberculosis strain SEA12334P6C4

Toxin (Protein)

• Gene name: phRel
• Uniprot ID: G0TIH2
• Locus tag: FPK09_RS07185
• Protein ID: WP_003407164.1
• Coordinates: 1547359..1548180 (+)
• Length: 274 a.a.

Antitoxin (Protein)

• Gene name: ATphRel
• Uniprot ID: A0A7U4BTV8
• Locus tag: FPK09_RS07190
• Protein ID: WP_003407167.1
• Coordinates: 1548173..1548409 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK09_RS07165	1542917..1543579	-	663	WP_003407152.1	membrane protein	-
FPK09_RS07170	1543576..1544361	-	786	WP_003407157.1	membrane protein	-
FPK09_RS07175	1544652..1546613	-	1962	WP_003407159.1	sigma-F factor regulator	-
FPK09_RS07180	1546752..1547138	-	387	WP_003898845.1	anti-sigma-F factor antagonist RsfA	-
FPK09_RS07185	1547359..1548180	+	822	WP_003407164.1	GTP pyrophosphokinase family protein	Toxin
FPK09_RS07190	1548173..1548409	+	237	WP_003407167.1	hypothetical protein	Antitoxin
FPK09_RS07195	1548481..1549686	-	1206	WP_003918234.1	serine hydrolase	-
FPK09_RS07200	1549989..1550774	+	786	WP_003407180.1	lipoprotein LprF	-
FPK09_RS07205	1550970..1552439	+	1470	WP_003900326.1	fatty acid desaturase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RelA/SpoT (76-174)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 274 a.a.
• Molecular weight: 30100.10 Da
• Isoelectric Point: 5.8187

>T129999 WP_003407164.1 NZ_CP041800:1547359-1548180 [Mycobacterium tuberculosis]
VVVALVGSAIVDLHSRPPWSNNAVRRLGVALRDGVDPPVDCPSYAEVMLWHADLAAEVQDRIEGRSWSASELLVTSRAKS
QDTLLAKLRRRPYLQLNTIQDIAGVRIDADLLLGEQTRLAREIADHFGADQPAIHDLRDHPHAGYRAVHVWLRLPAGRVE
IQIRTILQSLWANFYELLADAYGRGIRYDERPEQLAAGVVPAQLQELVGVMQDASADLAMHEAEWQHCAEIEYPGQRAMA
LGEASKNKATVLATTKFRLERAINEAESAGGGG

Nucleotide

• Length: 822 bp

>T129999 NZ_CP041800:1547359-1548180 [Mycobacterium tuberculosis]
GTGGTGGTAGCCCTCGTAGGCTCAGCAATCGTGGACTTGCATTCACGACCACCGTGGTCGAACAACGCGGTGCGTCGTCT
TGGCGTGGCACTGCGCGACGGAGTTGACCCGCCGGTCGACTGCCCGTCGTACGCCGAGGTGATGCTGTGGCATGCGGACT
TGGCCGCCGAAGTCCAGGACCGGATCGAGGGCCGGAGTTGGTCTGCGTCGGAGTTATTGGTTACCTCACGTGCGAAGAGC
CAAGACACCCTGCTAGCAAAGCTGCGGCGTCGGCCTTACCTGCAACTGAACACCATCCAAGACATCGCAGGTGTCCGCAT
CGATGCCGACCTCCTGCTGGGCGAGCAGACGAGACTTGCTCGCGAGATCGCCGACCACTTCGGTGCTGACCAGCCCGCTA
TTCATGATCTGCGTGACCACCCGCACGCCGGCTACCGGGCCGTTCATGTCTGGCTTCGGTTACCTGCCGGTCGTGTCGAG
ATACAGATTCGCACCATTTTGCAGAGCCTGTGGGCCAACTTCTACGAGCTTCTCGCTGACGCGTACGGTCGGGGCATCCG
CTATGACGAGCGGCCGGAGCAGCTAGCGGCCGGCGTTGTCCCGGCACAGCTTCAAGAGCTGGTAGGGGTTATGCAAGACG
CTTCAGCGGATCTGGCGATGCATGAAGCCGAGTGGCAACACTGTGCAGAGATCGAATACCCCGGCCAGCGGGCGATGGCG
CTTGGCGAGGCGAGCAAGAACAAGGCGACGGTGCTCGCAACGACCAAGTTTAGGCTGGAAAGGGCCATCAATGAGGCCGA
GTCGGCAGGGGGAGGTGGGTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8830.90 Da
• Isoelectric Point: 7.3178

>AT129999 WP_003407167.1 NZ_CP041800:1548173-1548409 [Mycobacterium tuberculosis]
VGEVAGYVVEYNRRTHVRRITEFATPQEAMEHRLKLEAERTDSNIEIVALVSKSLGTLKQTHSRYFTGEELNVGNGAR

Nucleotide

• Length: 237 bp

>AT129999 NZ_CP041800:1548173-1548409 [Mycobacterium tuberculosis]
GTGGGTGAGGTGGCTGGCTATGTCGTCGAATACAACCGGCGCACCCACGTGCGTCGCATCACCGAGTTCGCCACCCCGCA
AGAAGCGATGGAGCACCGGTTGAAGCTGGAAGCCGAGCGCACCGACAGCAATATCGAGATCGTTGCGCTCGTCAGTAAGT
CGTTGGGAACCCTGAAGCAAACGCATTCGCGGTACTTCACTGGTGAAGAGCTGAACGTCGGAAACGGCGCGCGGTAG

Structures

Toxin

• AlphaFold DB: G0TIH2

Antitoxin

• AlphaFold DB: A0A7U4BTV8