Detailed information of TA system TA129956

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 2905078..2905682
• Replicon: chromosome
• Accession: NZ_CP041799
• Organism: Mycobacterium tuberculosis strain SEA13020298P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: FPK10_RS13590
• Protein ID: WP_003414492.1
• Coordinates: 2905078..2905470 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A829CBY8
• Locus tag: FPK10_RS13595
• Protein ID: WP_003414495.1
• Coordinates: 2905467..2905682 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK10_RS13560	2900228..2901016	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
FPK10_RS13565	2901350..2901895	-	546	WP_014584866.1	DUF1802 family protein	-
FPK10_RS13570	2902167..2903051	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
FPK10_RS13575	2903054..2903941	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
FPK10_RS13580	2904246..2904791	-	546	WP_003910939.1	DUF1802 family protein	-
FPK10_RS13585	2904788..2905057	-	270	WP_003414489.1	DUF2277 family protein	-
FPK10_RS13590	2905078..2905470	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPK10_RS13595	2905467..2905682	-	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FPK10_RS13600	2905729..2906478	+	750	WP_003902358.1	enoyl-CoA hydratase	-
FPK10_RS13605	2906557..2907639	-	1083	WP_003414499.1	ABC transporter ATP-binding protein	-
FPK10_RS13610	2907632..2908942	-	1311	WP_003414501.1	ABC transporter substrate-binding protein	-
FPK10_RS13615	2908945..2909772	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
FPK10_RS13620	2909769..2910680	-	912	WP_003414505.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T129956 WP_003414492.1 NZ_CP041799:c2905470-2905078 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T129956 NZ_CP041799:c2905470-2905078 [Mycobacterium tuberculosis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7511.55 Da
• Isoelectric Point: 4.9732

>AT129956 WP_003414495.1 NZ_CP041799:c2905682-2905467 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT129956 NZ_CP041799:c2905682-2905467 [Mycobacterium tuberculosis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8

Antitoxin

• AlphaFold DB: A0A829CBY8