Detailed information of TA system TA129949

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2644192..2644829
• Replicon: chromosome
• Accession: NZ_CP041799
• Organism: Mycobacterium tuberculosis strain SEA13020298P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P0DMV7
• Locus tag: FPK10_RS12155
• Protein ID: WP_003413180.1
• Coordinates: 2644192..2644587 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ44
• Locus tag: FPK10_RS12160
• Protein ID: WP_003413183.1
• Coordinates: 2644584..2644829 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK10_RS12115	2639596..2640807	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
FPK10_RS12120	2640934..2641593	+	660	WP_003902296.1	hypothetical protein	-
FPK10_RS12125	2641590..2642252	+	663	WP_003905878.1	hypothetical protein	-
FPK10_RS12130	2642249..2642526	+	278	Protein_2400	type II toxin-antitoxin system VapB family antitoxin	-
FPK10_RS12135	2642619..2643032	+	414	WP_003413164.1	PIN domain nuclease	-
FPK10_RS12140	2643071..2643328	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	-
FPK10_RS12145	2643325..2643702	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	-
FPK10_RS12150	2643718..2644092	-	375	WP_003413177.1	hypothetical protein	-
FPK10_RS12155	2644192..2644587	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	Toxin
FPK10_RS12160	2644584..2644829	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	Antitoxin
FPK10_RS12165	2645240..2645659	-	420	WP_003413190.1	A24 family peptidase	-
FPK10_RS12170	2645671..2646480	-	810	WP_003413193.1	shikimate dehydrogenase	-
FPK10_RS12175	2646477..2647730	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
FPK10_RS12180	2647723..2648235	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endonuclease VapC20-like (1-129)

Antitoxin

• Ribbon-helix-helix protein, CopG (11-49)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14619.53 Da
• Isoelectric Point: 6.9794

>T129949 WP_003413180.1 NZ_CP041799:c2644587-2644192 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Nucleotide

• Length: 396 bp

>T129949 NZ_CP041799:c2644587-2644192 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9098.33 Da
• Isoelectric Point: 5.6007

>AT129949 WP_003413183.1 NZ_CP041799:c2644829-2644584 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Nucleotide

• Length: 246 bp

>AT129949 NZ_CP041799:c2644829-2644584 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Structures

Toxin

• PDB: 5WZ4
• PDB: 5WZF

Antitoxin

• AlphaFold DB: A0A7U4BW31