Detailed information of TA system TA129924

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 701551..702194
• Replicon: chromosome
• Accession: NZ_CP041799
• Organism: Mycobacterium tuberculosis strain SEA13020298P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P67239
• Locus tag: FPK10_RS03170
• Protein ID: WP_003403187.1
• Coordinates: 701793..702194 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ91
• Locus tag: FPK10_RS03165
• Protein ID: WP_003403184.1
• Coordinates: 701551..701796 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK10_RS03130	696831..697361	-	531	WP_003917320.1	two component system sensor kinase HK2	-
FPK10_RS03135	697345..698106	-	762	WP_003403148.1	two-component system response regulator TcrA	-
FPK10_RS03140	698163..698474	+	312	WP_003403164.1	hypothetical protein	-
FPK10_RS03145	698546..699496	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
FPK10_RS03150	699737..700321	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
FPK10_RS03155	700323..701033	+	711	Protein_625	transposase	-
FPK10_RS03160	701036..701506	+	471	WP_003898523.1	hypothetical protein	-
FPK10_RS03165	701551..701796	+	246	WP_003403184.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPK10_RS03170	701793..702194	+	402	WP_003403187.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPK10_RS03175	702550..703320	+	771	Protein_629	IS3-like element IS987 family transposase	-
FPK10_RS03180	703428..703649	+	222	WP_003906064.1	hypothetical protein	-
FPK10_RS03185	703744..704889	-	1146	WP_003417299.1	FHA domain-containing protein	-
FPK10_RS03190	705534..706598	+	1065	WP_003913037.1	GTP 3',8-cyclase MoaA	-
FPK10_RS03195	706695..707069	+	375	WP_003417295.1	4a-hydroxytetrahydrobiopterin dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	699737..703320	3583

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-80)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14501.21 Da
• Isoelectric Point: 4.4463

>T129924 WP_003403187.1 NZ_CP041799:701793-702194 [Mycobacterium tuberculosis]
VIVDTSAIIAILRDEDDAAAYADALANADVRRLSAASYLECGIVLDSQRDPVISRALDELIEEAEFVVEPVTERQARLAR
AAYADFGRGSGHPAGLNFGDCLSYALAIDRREPLLWKGNDFGHTGVQRALDRR

Nucleotide

• Length: 402 bp

>T129924 NZ_CP041799:701793-702194 [Mycobacterium tuberculosis]
GTGATCGTCGACACGTCGGCGATCATCGCGATTCTGCGCGACGAGGACGACGCCGCGGCCTACGCCGACGCGCTCGCCAA
CGCCGATGTCCGCAGACTGTCTGCGGCCAGCTACCTGGAATGCGGGATAGTCCTTGACTCCCAGCGTGATCCGGTCATCA
GCAGAGCACTGGATGAACTTATCGAAGAAGCCGAGTTCGTCGTCGAGCCGGTAACCGAGCGCCAGGCCCGCCTGGCCCGA
GCGGCCTACGCGGATTTCGGCAGAGGCAGCGGCCACCCCGCGGGCTTGAATTTCGGCGACTGCCTGTCCTATGCACTGGC
GATCGATCGACGTGAGCCGCTGCTGTGGAAGGGCAACGACTTTGGGCACACCGGCGTCCAAAGGGCACTGGATCGGCGGT
GA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8813.12 Da
• Isoelectric Point: 10.3486

>AT129924 WP_003403184.1 NZ_CP041799:701551-701796 [Mycobacterium tuberculosis]
MALNIKDPSVHQAVKQIAKITGESQARAVATAVNERLARLRSDDLAARLLAIGHKTASRMSPEAKRLDHDALLYDERGLP
A

Nucleotide

• Length: 246 bp

>AT129924 NZ_CP041799:701551-701796 [Mycobacterium tuberculosis]
ATGGCGTTGAACATCAAAGATCCGTCGGTTCACCAGGCGGTCAAGCAGATCGCGAAAATCACCGGCGAATCTCAGGCTCG
GGCGGTGGCGACCGCGGTGAACGAGCGTCTGGCCAGACTGCGCAGCGACGATCTCGCCGCCCGGCTCTTGGCTATCGGCC
ACAAGACCGCGAGCAGGATGAGCCCGGAAGCAAAGCGCCTCGACCACGATGCTCTGCTGTATGACGAGCGAGGGCTGCCG
GCGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSD6

Antitoxin

• AlphaFold DB: G0TQ91