Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3068176..3068863 Replicon chromosome
Accession NZ_CP041798
Organism Mycobacterium tuberculosis strain SEA13111P6C4

Toxin (Protein)


Gene name vapC Uniprot ID G0TF65
Locus tag FPK11_RS14365 Protein ID WP_003414064.1
Coordinates 3068468..3068863 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag FPK11_RS14360 Protein ID WP_003414061.1
Coordinates 3068176..3068442 (-) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPK11_RS14335 3063815..3064717 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
FPK11_RS14340 3064786..3065538 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
FPK11_RS14345 3065782..3066057 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
FPK11_RS14350 3066054..3067676 - 1623 WP_003414057.1 type I restriction-modification system subunit M -
FPK11_RS14355 3067763..3068179 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 -
FPK11_RS14360 3068176..3068442 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
FPK11_RS14365 3068468..3068863 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin Toxin
FPK11_RS14370 3068860..3069129 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
FPK11_RS14375 3069139..3070233 - 1095 WP_003414068.1 restriction endonuclease subunit S -
FPK11_RS14380 3070230..3070649 - 420 WP_003414070.1 winged helix-turn-helix domain-containing protein -
FPK11_RS14385 3070723..3071202 - 480 WP_003414073.1 dihydrofolate reductase -
FPK11_RS14390 3071273..3072073 - 801 WP_003911953.1 thymidylate synthase -
FPK11_RS14395 3072229..3072966 + 738 WP_003414079.1 dienelactone hydrolase family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-125)

Antitoxin

(16-56)


Sequences


Toxin        


Download         Length: 132 a.a.        Molecular weight: 14340.04 Da        Isoelectric Point: 4.7145

>T129904 WP_003414064.1 NZ_CP041798:c3068863-3068468 [Mycobacterium tuberculosis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Download         Length: 396 bp

>T129904 NZ_CP041798:c3068863-3068468 [Mycobacterium tuberculosis]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT129904 WP_003414061.1 NZ_CP041798:c3068442-3068176 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT129904 NZ_CP041798:c3068442-3068176 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB G0TF65


Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References