Detailed information of TA system TA129857

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0299-vapB/-
• Location: 366974..367545
• Replicon: chromosome
• Accession: NZ_CP041798
• Organism: Mycobacterium tuberculosis strain SEA13111P6C4

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: FPK11_RS01585
• Protein ID: WP_003401560.1
• Coordinates: 366974..367276 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: FPK11_RS01590
• Protein ID: WP_003401563.1
• Coordinates: 367324..367545 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK11_RS01565	362398..363201	-	804	WP_003401540.1	hypothetical protein	-
FPK11_RS01570	363211..364608	-	1398	WP_003401544.1	sulfatase	-
FPK11_RS01575	364787..366607	+	1821	WP_031653699.1	PE family protein	-
FPK11_RS01580	366750..366977	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
FPK11_RS01585	366974..367276	+	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
FPK11_RS01590	367324..367545	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
FPK11_RS01595	367542..367967	+	426	WP_003401566.1	PIN domain nuclease	-
FPK11_RS01600	368103..368735	+	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
FPK11_RS01605	368732..369640	+	909	WP_003900117.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T129857 WP_003401560.1 NZ_CP041798:366974-367276 [Mycobacterium tuberculosis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T129857 NZ_CP041798:366974-367276 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT129857 WP_003401563.1 NZ_CP041798:367324-367545 [Mycobacterium tuberculosis]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT129857 NZ_CP041798:367324-367545 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Antitoxin

• PDB: 3H87
• AlphaFold DB: A0A829CG48