Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2855880..2856517 Replicon chromosome
Accession NZ_CP041797
Organism Mycobacterium tuberculosis strain SEA14117P6C4

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag FPK12_RS13275 Protein ID WP_003413180.1
Coordinates 2855880..2856275 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag FPK12_RS13280 Protein ID WP_003413183.1
Coordinates 2856272..2856517 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPK12_RS13235 2851283..2852494 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
FPK12_RS13240 2852621..2853280 + 660 WP_003900846.1 LppA family lipoprotein -
FPK12_RS13245 2853277..2853939 + 663 WP_003901421.1 hypothetical protein -
FPK12_RS13250 2853936..2854214 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
FPK12_RS13255 2854307..2854720 + 414 WP_003413164.1 PIN domain nuclease -
FPK12_RS13260 2854759..2855016 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
FPK12_RS13265 2855013..2855390 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
FPK12_RS13270 2855406..2855780 - 375 WP_003413177.1 hypothetical protein -
FPK12_RS13275 2855880..2856275 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
FPK12_RS13280 2856272..2856517 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
FPK12_RS13285 2856928..2857347 - 420 WP_003413190.1 A24 family peptidase -
FPK12_RS13290 2857359..2858168 - 810 WP_003413193.1 shikimate dehydrogenase -
FPK12_RS13295 2858165..2859418 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
FPK12_RS13300 2859411..2859923 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T129840 WP_003413180.1 NZ_CP041797:c2856275-2855880 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T129840 NZ_CP041797:c2856275-2855880 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT129840 WP_003413183.1 NZ_CP041797:c2856517-2856272 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT129840 NZ_CP041797:c2856517-2856272 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References