Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2854759..2855390 Replicon chromosome
Accession NZ_CP041797
Organism Mycobacterium tuberculosis strain SEA14117P6C4

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag FPK12_RS13265 Protein ID WP_003413174.1
Coordinates 2855013..2855390 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag FPK12_RS13260 Protein ID WP_003413167.1
Coordinates 2854759..2855016 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPK12_RS13230 2850673..2850987 + 315 WP_009937839.1 hypothetical protein -
FPK12_RS13235 2851283..2852494 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
FPK12_RS13240 2852621..2853280 + 660 WP_003900846.1 LppA family lipoprotein -
FPK12_RS13245 2853277..2853939 + 663 WP_003901421.1 hypothetical protein -
FPK12_RS13250 2853936..2854214 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
FPK12_RS13255 2854307..2854720 + 414 WP_003413164.1 PIN domain nuclease -
FPK12_RS13260 2854759..2855016 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein Antitoxin
FPK12_RS13265 2855013..2855390 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
FPK12_RS13270 2855406..2855780 - 375 WP_003413177.1 hypothetical protein -
FPK12_RS13275 2855880..2856275 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
FPK12_RS13280 2856272..2856517 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
FPK12_RS13285 2856928..2857347 - 420 WP_003413190.1 A24 family peptidase -
FPK12_RS13290 2857359..2858168 - 810 WP_003413193.1 shikimate dehydrogenase -
FPK12_RS13295 2858165..2859418 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
FPK12_RS13300 2859411..2859923 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T129839 WP_003413174.1 NZ_CP041797:2855013-2855390 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T129839 NZ_CP041797:2855013-2855390 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT129839 WP_003413167.1 NZ_CP041797:2854759-2855016 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT129839 NZ_CP041797:2854759-2855016 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References