Detailed information of TA system TA129764

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2196926..2197629
• Replicon: chromosome
• Accession: NZ_CP041796
• Organism: Mycobacterium tuberculosis strain SEA14318P6C4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: FPK13_RS10055
• Protein ID: WP_003409778.1
• Coordinates: 2196926..2197255 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: FPK13_RS10060
• Protein ID: WP_003409780.1
• Coordinates: 2197252..2197629 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK13_RS10035	2193309..2194379	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
FPK13_RS10040	2194376..2194891	+	516	WP_031699471.1	flavin reductase family protein	-
FPK13_RS10045	2194888..2195949	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
FPK13_RS10050	2195946..2196716	+	771	WP_003409775.1	SDR family oxidoreductase	-
FPK13_RS10055	2196926..2197255	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FPK13_RS10060	2197252..2197629	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
FPK13_RS10065	2197626..2198216	-	591	WP_003409784.1	SEC-C domain-containing protein	-
FPK13_RS10070	2198271..2199635	+	1365	WP_031648120.1	HNH endonuclease	-
FPK13_RS10075	2199790..2200242	-	453	WP_003899095.1	lipoprotein	-
FPK13_RS10080	2200306..2200707	+	402	WP_003409869.1	hypothetical protein	-
FPK13_RS10085	2200700..2200882	-	183	WP_003409870.1	hypothetical protein	-
FPK13_RS10090	2200996..2201346	-	351	WP_003409871.1	hypothetical protein	-
FPK13_RS10095	2201357..2202259	-	903	WP_003409874.1	hypothetical protein	-
FPK13_RS10100	2202280..2202471	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T129764 WP_003409778.1 NZ_CP041796:c2197255-2196926 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T129764 NZ_CP041796:c2197255-2196926 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT129764 WP_003409780.1 NZ_CP041796:c2197629-2197252 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT129764 NZ_CP041796:c2197629-2197252 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8