Detailed information of TA system TA129755

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1240615..1241183
• Replicon: chromosome
• Accession: NZ_CP041796
• Organism: Mycobacterium tuberculosis strain SEA14318P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: FPK13_RS05850
• Protein ID: WP_003405865.1
• Coordinates: 1240809..1241183 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: FPK13_RS05845
• Protein ID: WP_003405863.1
• Coordinates: 1240615..1240812 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK13_RS05825	1236656..1237294	-	639	WP_003898730.1	lipid droplet-associated protein	-
FPK13_RS05830	1237384..1238391	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
FPK13_RS05835	1238408..1239391	-	984	WP_003903326.1	hypothetical protein	-
FPK13_RS05840	1239454..1240527	+	1074	WP_031699318.1	redox-regulated ATPase YchF	-
FPK13_RS05845	1240615..1240812	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPK13_RS05850	1240809..1241183	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPK13_RS05855	1241386..1242084	+	699	WP_003898733.1	hypothetical protein	-
FPK13_RS05860	1242202..1242387	+	186	WP_003901093.1	hypothetical protein	-
FPK13_RS05865	1242314..1242589	-	276	WP_003405867.1	hypothetical protein	-
FPK13_RS05870	1242832..1243155	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
FPK13_RS05875	1243170..1243880	-	711	WP_003911399.1	hypothetical protein	-
FPK13_RS05880	1243902..1244111	+	210	WP_003911400.1	hypothetical protein	-
FPK13_RS05885	1244063..1244770	-	708	Protein_1159	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T129755 WP_003405865.1 NZ_CP041796:1240809-1241183 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T129755 NZ_CP041796:1240809-1241183 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT129755 WP_003405863.1 NZ_CP041796:1240615-1240812 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT129755 NZ_CP041796:1240615-1240812 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07