Detailed information of TA system TA129699

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 1579039..1579694
• Replicon: chromosome
• Accession: NZ_CP041795
• Organism: Mycobacterium tuberculosis strain SEA14333P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FPK14_RS07320
• Protein ID: WP_193019842.1
• Coordinates: 1579039..1579440 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TIK2
• Locus tag: FPK14_RS07325
• Protein ID: WP_003407272.1
• Coordinates: 1579437..1579694 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK14_RS07305	1574512..1575897	-	1386	WP_003407260.1	cytochrome P450	-
FPK14_RS07310	1575975..1577009	+	1035	WP_003407264.1	AraC family transcriptional regulator	-
FPK14_RS07315	1577055..1578784	-	1730	Protein_1440	PE family protein	-
FPK14_RS07320	1579039..1579440	-	402	WP_193019842.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPK14_RS07325	1579437..1579694	-	258	WP_003407272.1	ribbon-helix-helix domain-containing protein	Antitoxin
FPK14_RS07330	1579777..1580736	-	960	WP_003908687.1	alpha/beta hydrolase	-
FPK14_RS07335	1580761..1581723	-	963	WP_003407279.1	alpha/beta hydrolase	-
FPK14_RS07340	1581722..1582459	+	738	WP_193019858.1	lysoplasmalogenase	-
FPK14_RS07345	1582540..1584507	+	1968	WP_003407285.1	primosomal protein N'	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-108)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-39)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14922.41 Da
• Isoelectric Point: 11.8222

>T129699 WP_193019842.1 NZ_CP041795:c1579440-1579039 [Mycobacterium tuberculosis]
MILVDSDVLIAHLRGVVAARDWLVSARRDGPLAISVVSTAELIGGMRTAERREVWRLLASFRVQPATEVIARRAGDMMRR
YRRSHNRIGLGDYLIAATADVQGLQLATLNVWHFPMFEQLKPPFAVPGHRPRA

Nucleotide

• Length: 402 bp

>T129699 NZ_CP041795:c1579440-1579039 [Mycobacterium tuberculosis]
ATGATCCTTGTCGACTCCGATGTGCTGATCGCGCATTTGCGGGGTGTCGTTGCTGCTCGCGATTGGCTTGTCAGCGCCCG
CAGGGACGGACCGCTGGCGATCAGCGTGGTGTCCACCGCCGAACTCATCGGCGGAATGCGGACCGCCGAACGGCGCGAGG
TGTGGCGCCTGCTTGCATCGTTTCGGGTACAGCCAGCAACCGAGGTAATCGCACGCCGCGCCGGCGACATGATGCGCCGA
TATCGTCGCAGCCACAACCGGATTGGACTTGGCGACTATCTGATAGCTGCTACCGCCGACGTCCAGGGCCTGCAATTGGC
AACCCTCAACGTGTGGCATTTCCCCATGTTCGAGCAGCTGAAACCACCATTTGCGGTGCCGGGGCACCGACCGCGGGCAT
GA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9443.58 Da
• Isoelectric Point: 6.4631

>AT129699 WP_003407272.1 NZ_CP041795:c1579694-1579437 [Mycobacterium tuberculosis]
MKRTNIYLDEEQTASLDKLAAQEGVSRAELIRLLLNRALTTAGDDLASDLQAINDSFGTLRHLDPPVRRSGGREQHLAQV
WRATS

Nucleotide

• Length: 258 bp

>AT129699 NZ_CP041795:c1579694-1579437 [Mycobacterium tuberculosis]
ATGAAGCGGACCAACATCTACCTCGACGAGGAGCAGACGGCAAGCCTCGACAAGTTGGCCGCGCAAGAAGGTGTTTCGCG
CGCCGAGCTGATCCGCCTCCTGCTGAACCGAGCCCTCACCACCGCTGGGGACGACCTTGCATCGGACCTGCAGGCTATAA
ACGATTCGTTCGGCACGCTTCGCCACCTGGATCCGCCGGTGCGTCGCTCCGGTGGTCGTGAACAGCACCTTGCCCAGGTG
TGGCGCGCCACCTCATGA

Structures

Antitoxin

• AlphaFold DB: G0TIK2