Detailed information of TA system TA129676

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0299-vapB/-
• Location: 366788..367359
• Replicon: chromosome
• Accession: NZ_CP041795
• Organism: Mycobacterium tuberculosis strain SEA14333P6C4

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: FPK14_RS01590
• Protein ID: WP_003401560.1
• Coordinates: 366788..367090 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: FPK14_RS01595
• Protein ID: WP_003401563.1
• Coordinates: 367138..367359 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK14_RS01570	362212..363015	-	804	WP_003401540.1	hypothetical protein	-
FPK14_RS01575	363025..364422	-	1398	WP_003401544.1	sulfatase	-
FPK14_RS01580	364601..366421	+	1821	WP_031653699.1	PE family protein	-
FPK14_RS01585	366564..366791	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
FPK14_RS01590	366788..367090	+	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
FPK14_RS01595	367138..367359	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
FPK14_RS01600	367356..367781	+	426	WP_003401566.1	PIN domain nuclease	-
FPK14_RS01605	367917..368549	+	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
FPK14_RS01610	368546..369454	+	909	WP_003900117.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T129676 WP_003401560.1 NZ_CP041795:366788-367090 [Mycobacterium tuberculosis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T129676 NZ_CP041795:366788-367090 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT129676 WP_003401563.1 NZ_CP041795:367138-367359 [Mycobacterium tuberculosis]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT129676 NZ_CP041795:367138-367359 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Antitoxin

• PDB: 3H87
• AlphaFold DB: A0A829CG48