Detailed information of TA system TA129660

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2876340..2876977
• Replicon: chromosome
• Accession: NZ_CP041794
• Organism: Mycobacterium tuberculosis strain SEA15228P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FPK16_RS13330
• Protein ID: WP_193019429.1
• Coordinates: 2876340..2876735 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ44
• Locus tag: FPK16_RS13335
• Protein ID: WP_003413183.1
• Coordinates: 2876732..2876977 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK16_RS13285	2871769..2872428	+	660	WP_003900846.1	LppA family lipoprotein	-
FPK16_RS13290	2872425..2873084	+	660	WP_003900847.1	hypothetical protein	-
FPK16_RS13295	2873081..2873740	+	660	WP_193019428.1	hypothetical protein	-
FPK16_RS13300	2873737..2874399	+	663	WP_055351404.1	hypothetical protein	-
FPK16_RS13305	2874396..2874674	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
FPK16_RS13310	2874767..2875180	+	414	WP_003413164.1	PIN domain nuclease	-
FPK16_RS13315	2875219..2875476	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	-
FPK16_RS13320	2875473..2875850	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	-
FPK16_RS13325	2875866..2876240	-	375	WP_003413177.1	hypothetical protein	-
FPK16_RS13330	2876340..2876735	-	396	WP_193019429.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	Toxin
FPK16_RS13335	2876732..2876977	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	Antitoxin
FPK16_RS13340	2877388..2877807	-	420	WP_003413190.1	A24 family peptidase	-
FPK16_RS13345	2877819..2878628	-	810	WP_003413193.1	shikimate dehydrogenase	-
FPK16_RS13350	2878625..2879881	-	1257	WP_014585306.1	endolytic transglycosylase MltG	-
FPK16_RS13355	2879874..2880386	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endonuclease VapC20-like (1-129)

Antitoxin

• Ribbon-helix-helix protein, CopG (11-49)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14647.58 Da
• Isoelectric Point: 6.9794

>T129660 WP_193019429.1 NZ_CP041794:c2876735-2876340 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDVTSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Nucleotide

• Length: 396 bp

>T129660 NZ_CP041794:c2876735-2876340 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGTCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9098.33 Da
• Isoelectric Point: 5.6007

>AT129660 WP_003413183.1 NZ_CP041794:c2876977-2876732 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Nucleotide

• Length: 246 bp

>AT129660 NZ_CP041794:c2876977-2876732 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Structures

Antitoxin

• AlphaFold DB: A0A7U4BW31