Detailed information of TA system TA129620

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 644727..645403
• Replicon: chromosome
• Accession: NZ_CP041794
• Organism: Mycobacterium tuberculosis strain SEA15228P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TPQ9
• Locus tag: FPK16_RS02870
• Protein ID: WP_003898500.1
• Coordinates: 644727..645140 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TPR0
• Locus tag: FPK16_RS02875
• Protein ID: WP_003402915.1
• Coordinates: 645137..645403 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK16_RS02840	640072..640374	-	303	WP_003402896.1	DUF3349 domain-containing protein	-
FPK16_RS02845	640434..640712	-	279	WP_003402901.1	hypothetical protein	-
FPK16_RS02850	640709..641962	-	1254	WP_003402904.1	inorganic phosphate transporter family protein	-
FPK16_RS02855	642082..642468	-	387	WP_003402907.1	VOC family protein	-
FPK16_RS02860	642531..643415	-	885	WP_003402909.1	SDR family oxidoreductase	-
FPK16_RS02865	643511..644413	-	903	WP_003915439.1	1,4-dihydroxy-2-naphthoyl-CoA synthase	-
FPK16_RS02870	644727..645140	-	414	WP_003898500.1	PIN domain-containing protein	Toxin
FPK16_RS02875	645137..645403	-	267	WP_003402915.1	antitoxin VapB3	Antitoxin
FPK16_RS02880	645595..647310	-	1716	WP_193019490.1	fatty-acid--CoA ligase FadD8	-
FPK16_RS02885	647388..648992	+	1605	WP_061901081.1	amidohydrolase	-
FPK16_RS02890	648989..649969	+	981	WP_003402923.1	o-succinylbenzoate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (13-129)

Antitoxin

• Post-segregation antitoxin CcdA (19-61)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14667.78 Da
• Isoelectric Point: 7.3603

>T129620 WP_003898500.1 NZ_CP041794:c645140-644727 [Mycobacterium tuberculosis]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Nucleotide

• Length: 414 bp

>T129620 NZ_CP041794:c645140-644727 [Mycobacterium tuberculosis]
GTGAGAGCATCGCCCACTTCGCCGCCGGAGCAGGTGGTCGTCGACGCGAGTGCCATGGTGGATCTACTGGCTCGCACTAG
CGATCGGTGCTCTGCGGTGCGCGCGCGGCTGGCTCGGACCGCGATGCACGCGCCGGCGCACTTCGATGCAGAGGTGTTGT
CGGCGCTGGGGCGCATGCAGCGCGCCGGCGCACTCACCGTTGCCTATGTCGATGCGGCACTGGAGGAGTTGCGACAGGTG
CCGGTGACTCGACACGGTCTTTCGTCGCTGCTTGCTGGAGCGTGGTCGCGCCGCGACACCCTCCGCCTGACCGATGCCCT
CTACGTCGAGCTGGCCGAAACGGCAGGTCTGGTGTTGTTGACCACCGACGAAAGATTGGCACGCGCCTGGCCCTCGGCTC
ACGCCATCGGCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9543.79 Da
• Isoelectric Point: 4.5994

>AT129620 WP_003402915.1 NZ_CP041794:c645403-645137 [Mycobacterium tuberculosis]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Nucleotide

• Length: 267 bp

>AT129620 NZ_CP041794:c645403-645137 [Mycobacterium tuberculosis]
TTGTTGAGCCGGCGCACGAAAACGATCGTTGTGTGTACATTGGTGTGTATGGCTCGGTTGAACGTGTATGTGCCCGACGA
ATTGGCGGAGCGCGCCAGGGCGCGGGGCTTGAACGTCTCGGCGCTGACTCAGGCCGCGATCAGTGCCGAGTTGGAGAACT
CCGCAACCGATGCGTGGCTTGAGGGGTTGGAACCCAGAAGCACCGGCGCTCGGCATGATGACGTGCTGGGTGCGATCGAT
GCCGCTCGCGATGAGTTCGAAGCGTGA

Structures

Toxin

• AlphaFold DB: G0TPQ9

Antitoxin

• AlphaFold DB: G0TPR0