Detailed information of TA system TA129563

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 701422..702068
• Replicon: chromosome
• Accession: NZ_CP041793
• Organism: Mycobacterium tuberculosis strain SEA15229P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: FPK17_RS03125
• Protein ID: WP_003403137.1
• Coordinates: 701422..701835 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: FPK17_RS03130
• Protein ID: WP_003403139.1
• Coordinates: 701832..702068 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK17_RS03105	697505..699055	+	1551	WP_003403119.1	MCE family protein	-
FPK17_RS03110	699107..699499	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
FPK17_RS03115	699496..699753	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
FPK17_RS03120	699936..701171	-	1236	WP_003403128.1	ATP-binding protein	-
FPK17_RS03125	701422..701835	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPK17_RS03130	701832..702068	-	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FPK17_RS03135	702172..702678	-	507	WP_003900973.1	two component system sensor kinase HK1	-
FPK17_RS03140	702792..703322	-	531	WP_003917320.1	two component system sensor kinase HK2	-
FPK17_RS03145	703306..704067	-	762	WP_003904438.1	two-component system response regulator TcrA	-
FPK17_RS03150	704124..704435	+	312	WP_003403164.1	hypothetical protein	-
FPK17_RS03155	704507..705457	+	951	WP_003907436.1	DUF1259 domain-containing protein	-
FPK17_RS03160	705698..706282	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
FPK17_RS03165	706284..706994	+	711	Protein_627	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T129563 WP_003403137.1 NZ_CP041793:c701835-701422 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T129563 NZ_CP041793:c701835-701422 [Mycobacterium tuberculosis]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT129563 WP_003403139.1 NZ_CP041793:c702068-701832 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT129563 NZ_CP041793:c702068-701832 [Mycobacterium tuberculosis]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89