Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3107077..3107647 Replicon chromosome
Accession NZ_CP041792
Organism Mycobacterium tuberculosis strain SEA15230P6C4

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag FPK18_RS14595 Protein ID WP_003414166.1
Coordinates 3107077..3107433 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag FPK18_RS14600 Protein ID WP_003901465.1
Coordinates 3107417..3107647 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPK18_RS14575 3102526..3104214 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
FPK18_RS14580 3104218..3104544 - 327 WP_003414157.1 hypothetical protein -
FPK18_RS14585 3104717..3105307 + 591 WP_031656198.1 DUF3558 family protein -
FPK18_RS14590 3105326..3106975 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
FPK18_RS14595 3107077..3107433 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
FPK18_RS14600 3107417..3107647 - 231 WP_003901465.1 antitoxin MazE Antitoxin
FPK18_RS14605 3107690..3108733 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
FPK18_RS14610 3108924..3109199 + 276 WP_003911993.1 DUF1778 domain-containing protein -
FPK18_RS14615 3109375..3109629 - 255 WP_003917684.1 hypothetical protein -
FPK18_RS14620 3109777..3110181 + 405 WP_003414181.1 hypothetical protein -
FPK18_RS14625 3110178..3110369 + 192 WP_003414184.1 hypothetical protein -
FPK18_RS14630 3110433..3111722 + 1290 Protein_2887 transposase family protein -
FPK18_RS14635 3111956..3112213 + 258 WP_003899489.1 hypothetical protein -
FPK18_RS14640 3112318..3112629 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T129545 WP_003414166.1 NZ_CP041792:c3107433-3107077 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T129545 NZ_CP041792:c3107433-3107077 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT129545 WP_003901465.1 NZ_CP041792:c3107647-3107417 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT129545 NZ_CP041792:c3107647-3107417 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References