Detailed information of TA system TA129541

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2877541..2878178
• Replicon: chromosome
• Accession: NZ_CP041792
• Organism: Mycobacterium tuberculosis strain SEA15230P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P0DMV7
• Locus tag: FPK18_RS13360
• Protein ID: WP_003413180.1
• Coordinates: 2877541..2877936 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ44
• Locus tag: FPK18_RS13365
• Protein ID: WP_003413183.1
• Coordinates: 2877933..2878178 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK18_RS13320	2873626..2874285	+	660	WP_003900846.1	LppA family lipoprotein	-
FPK18_RS13325	2874282..2874941	+	660	WP_003900847.1	hypothetical protein	-
FPK18_RS13330	2874938..2875600	+	663	WP_003900848.1	hypothetical protein	-
FPK18_RS13335	2875597..2875875	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
FPK18_RS13340	2875968..2876381	+	414	WP_003413164.1	PIN domain nuclease	-
FPK18_RS13345	2876420..2876677	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	-
FPK18_RS13350	2876674..2877051	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	-
FPK18_RS13355	2877067..2877441	-	375	WP_003413177.1	hypothetical protein	-
FPK18_RS13360	2877541..2877936	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	Toxin
FPK18_RS13365	2877933..2878178	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	Antitoxin
FPK18_RS13370	2878589..2879008	-	420	WP_003413190.1	A24 family peptidase	-
FPK18_RS13375	2879020..2879829	-	810	WP_003413193.1	shikimate dehydrogenase	-
FPK18_RS13380	2879826..2881079	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
FPK18_RS13385	2881072..2881584	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endonuclease VapC20-like (1-129)

Antitoxin

• Ribbon-helix-helix protein, CopG (11-49)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14619.53 Da
• Isoelectric Point: 6.9794

>T129541 WP_003413180.1 NZ_CP041792:c2877936-2877541 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Nucleotide

• Length: 396 bp

>T129541 NZ_CP041792:c2877936-2877541 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9098.33 Da
• Isoelectric Point: 5.6007

>AT129541 WP_003413183.1 NZ_CP041792:c2878178-2877933 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Nucleotide

• Length: 246 bp

>AT129541 NZ_CP041792:c2878178-2877933 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Structures

Toxin

• PDB: 5WZ4
• PDB: 5WZF

Antitoxin

• AlphaFold DB: A0A7U4BW31