Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2246955..2247541 Replicon chromosome
Accession NZ_CP041792
Organism Mycobacterium tuberculosis strain SEA15230P6C4

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag FPK18_RS10415 Protein ID WP_003410010.1
Coordinates 2246955..2247299 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag FPK18_RS10420 Protein ID WP_003410014.1
Coordinates 2247293..2247541 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPK18_RS10380 2242661..2243260 + 600 WP_003409989.1 L-lysine exporter -
FPK18_RS10385 2243676..2244104 + 429 WP_003409992.1 cellulose-binding protein -
FPK18_RS10390 2244330..2244869 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
FPK18_RS10395 2245389..2245949 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
FPK18_RS10400 2245946..2246287 - 342 WP_003410003.1 DUF2384 domain-containing protein -
FPK18_RS10405 2246373..2246630 + 258 WP_003410006.1 hypothetical protein -
FPK18_RS10410 2246531..2246866 - 336 WP_003410009.1 dehydrogenase -
FPK18_RS10415 2246955..2247299 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
FPK18_RS10420 2247293..2247541 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
FPK18_RS10425 2247641..2249956 - 2316 WP_129446508.1 cation transporter ATPase CptG -
FPK18_RS10430 2249953..2250225 - 273 WP_003903706.1 DUF1490 family protein -
FPK18_RS10435 2250278..2250634 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
FPK18_RS10440 2250791..2251132 + 342 Protein_2063 hemerythrin domain-containing protein -
FPK18_RS10445 2251135..2251767 - 633 Protein_2064 amino acid permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T129531 WP_003410010.1 NZ_CP041792:c2247299-2246955 [Mycobacterium tuberculosis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T129531 NZ_CP041792:c2247299-2246955 [Mycobacterium tuberculosis]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT129531 WP_003410014.1 NZ_CP041792:c2247541-2247293 [Mycobacterium tuberculosis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT129531 NZ_CP041792:c2247541-2247293 [Mycobacterium tuberculosis]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References