Detailed information of TA system TA129523

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1766257..1766870
• Replicon: chromosome
• Accession: NZ_CP041792
• Organism: Mycobacterium tuberculosis strain SEA15230P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FPK18_RS08165
• Protein ID: WP_129446556.1
• Coordinates: 1766466..1766870 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0E8UJ46
• Locus tag: FPK18_RS08160
• Protein ID: WP_009935474.1
• Coordinates: 1766257..1766460 (+)
• Length: 68 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK18_RS08145	1761521..1764424	+	2904	WP_003904644.1	MMPL family transporter	-
FPK18_RS08150	1764434..1764880	+	447	WP_003407780.1	F420H(2)-dependent quinone reductase	-
FPK18_RS08155	1764915..1766204	+	1290	WP_003407781.1	threonine ammonia-lyase	-
FPK18_RS08160	1766257..1766460	+	204	WP_009935474.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPK18_RS08165	1766466..1766870	+	405	WP_129446556.1	type II toxin-antitoxin system toxin ribonuclease VapC11	Toxin
FPK18_RS08170	1766887..1768627	-	1741	Protein_1611	malto-oligosyltrehalose trehalohydrolase	-
FPK18_RS08175	1768620..1770917	-	2298	WP_003407790.1	malto-oligosyltrehalose synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14607.73 Da
• Isoelectric Point: 5.1784

>T129523 WP_129446556.1 NZ_CP041792:1766466-1766870 [Mycobacterium tuberculosis]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIALITNLQAASFR

Nucleotide

• Length: 405 bp

>T129523 NZ_CP041792:1766466-1766870 [Mycobacterium tuberculosis]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCTGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin

Protein

• Length: 68 a.a.
• Molecular weight: 7501.48 Da
• Isoelectric Point: 4.3951

>AT129523 WP_009935474.1 NZ_CP041792:1766257-1766460 [Mycobacterium tuberculosis]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Nucleotide

• Length: 204 bp

>AT129523 NZ_CP041792:1766257-1766460 [Mycobacterium tuberculosis]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E8UJ46