Detailed information of TA system TA129514

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1028422..1029395
• Replicon: chromosome
• Accession: NZ_CP041792
• Organism: Mycobacterium tuberculosis strain SEA15230P6C4

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: O05911
• Locus tag: FPK18_RS04830
• Protein ID: WP_003404756.1
• Coordinates: 1028895..1029395 (+)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: G0TFR4
• Locus tag: FPK18_RS04825
• Protein ID: WP_003404754.1
• Coordinates: 1028422..1028898 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK18_RS04805	1024269..1025540	-	1272	WP_193018003.1	PPE family protein	-
FPK18_RS04810	1025555..1025854	-	300	WP_003404748.1	PE family protein	-
FPK18_RS04815	1026298..1028079	+	1782	WP_003903231.1	BCCT family transporter	-
FPK18_RS04820	1028076..1028333	+	258	WP_003404750.1	hypothetical protein	-
FPK18_RS04825	1028422..1028898	+	477	WP_003404754.1	DUF1778 domain-containing protein	Antitoxin
FPK18_RS04830	1028895..1029395	+	501	WP_003404756.1	GNAT family N-acetyltransferase	Toxin
FPK18_RS04840	1029708..1031027	-	1320	WP_003404757.1	IS256-like element IS1554 family transposase	-
FPK18_RS04845	1031315..1031896	+	582	WP_003904507.1	IS607-like element IS1535 family transposase	-
FPK18_RS04850	1031896..1033548	+	1653	WP_003903232.1	IS607 family element transposase accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (38-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (74-150)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18048.55 Da
• Isoelectric Point: 7.8659

>T129514 WP_003404756.1 NZ_CP041792:1028895-1029395 [Mycobacterium tuberculosis]
VSGYSAPRRISDADDVTSFSSGEPSLDDYLRKRALANHVQGGSRCFVTCRDGRVVGFYALASGSVAHADAPGRVRRNMPD
PVPVILLSRLAVDRKEQGRGLGSHLLRDAIGRCVQAADSIGLRAILVHALHDEARAFYVHFDFEISPTDPLHLMLLMKDA
RALIGD

Nucleotide

• Length: 501 bp

>T129514 NZ_CP041792:1028895-1029395 [Mycobacterium tuberculosis]
GTGAGCGGCTACAGCGCGCCGCGACGTATCAGCGACGCCGATGACGTCACGAGCTTCAGCAGCGGCGAGCCCAGTCTGGA
CGATTACTTGCGCAAGCGGGCGTTGGCCAACCATGTGCAGGGAGGGTCGCGCTGTTTCGTGACGTGCCGTGACGGTCGGG
TAGTCGGCTTCTATGCGCTAGCGTCAGGGTCGGTCGCACACGCTGATGCTCCGGGACGGGTGCGCCGCAATATGCCTGAC
CCCGTGCCGGTGATCCTGCTGTCGCGGTTGGCGGTTGATCGCAAAGAACAGGGCAGGGGCCTGGGCAGTCATCTGCTGCG
TGATGCGATCGGTCGCTGTGTCCAGGCTGCGGACTCGATCGGGCTGCGGGCGATTCTTGTTCATGCGTTGCACGATGAGG
CCCGCGCGTTCTACGTCCACTTCGACTTCGAGATCTCGCCGACCGATCCGCTGCACCTAATGCTGTTGATGAAAGACGCT
CGCGCGCTAATTGGCGACTGA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 17357.95 Da
• Isoelectric Point: 11.2467

>AT129514 WP_003404754.1 NZ_CP041792:1028422-1028898 [Mycobacterium tuberculosis]
MHRAGAAVTANVWCRAGGIRMAPRPVIPVATQQRLRRQADRQSLGGSGLPALNCTPIRHTIDVMATKPERKTERLAARLT
PEQDALIRRAAEAEGTDLTNFTVTAALAHARDVLADRRLFVLTDAAWTEFLAALDRPVSHKPRLEKLFAARSIFDTEG

Nucleotide

• Length: 477 bp

>AT129514 NZ_CP041792:1028422-1028898 [Mycobacterium tuberculosis]
TTGCACCGAGCGGGAGCGGCGGTGACGGCCAACGTGTGGTGTCGTGCGGGCGGCATTCGGATGGCGCCACGGCCGGTCAT
CCCGGTGGCTACGCAGCAGCGCCTGCGGCGGCAGGCGGATCGCCAGAGCCTGGGTGGTAGCGGCTTGCCAGCGTTGAATT
GTACGCCTATCAGGCACACAATTGATGTCATGGCTACCAAGCCTGAGCGGAAGACCGAGCGTCTTGCAGCGCGCCTGACC
CCTGAGCAGGACGCGCTGATTCGTCGTGCTGCCGAGGCCGAGGGGACTGACCTCACCAATTTCACGGTTACAGCGGCGTT
GGCGCACGCGCGCGACGTGCTGGCCGACCGCCGGCTCTTCGTACTCACCGATGCCGCGTGGACTGAGTTCCTCGCCGCGC
TGGACCGGCCCGTCTCACACAAGCCTCGGTTGGAGAAGCTGTTCGCCGCGCGGTCCATTTTCGACACCGAGGGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSZ2

Antitoxin

• AlphaFold DB: G0TFR4