Detailed information of TA system TA129500

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 644761..645437
• Replicon: chromosome
• Accession: NZ_CP041792
• Organism: Mycobacterium tuberculosis strain SEA15230P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TPQ9
• Locus tag: FPK18_RS02875
• Protein ID: WP_003898500.1
• Coordinates: 644761..645174 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TPR0
• Locus tag: FPK18_RS02880
• Protein ID: WP_003402915.1
• Coordinates: 645171..645437 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK18_RS02845	640106..640408	-	303	WP_003402896.1	DUF3349 domain-containing protein	-
FPK18_RS02850	640468..640746	-	279	WP_003402901.1	hypothetical protein	-
FPK18_RS02855	640743..641996	-	1254	WP_003402904.1	inorganic phosphate transporter family protein	-
FPK18_RS02860	642116..642502	-	387	WP_003402907.1	VOC family protein	-
FPK18_RS02865	642565..643449	-	885	WP_003402909.1	SDR family oxidoreductase	-
FPK18_RS02870	643545..644447	-	903	WP_003915439.1	1,4-dihydroxy-2-naphthoyl-CoA synthase	-
FPK18_RS02875	644761..645174	-	414	WP_003898500.1	PIN domain-containing protein	Toxin
FPK18_RS02880	645171..645437	-	267	WP_003402915.1	antitoxin VapB3	Antitoxin
FPK18_RS02885	645629..647344	-	1716	WP_003402917.1	fatty-acid--CoA ligase FadD8	-
FPK18_RS02890	647422..649026	+	1605	WP_003903056.1	amidohydrolase	-
FPK18_RS02895	649023..650003	+	981	WP_003402923.1	o-succinylbenzoate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (13-129)

Antitoxin

• Post-segregation antitoxin CcdA (19-61)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14667.78 Da
• Isoelectric Point: 7.3603

>T129500 WP_003898500.1 NZ_CP041792:c645174-644761 [Mycobacterium tuberculosis]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Nucleotide

• Length: 414 bp

>T129500 NZ_CP041792:c645174-644761 [Mycobacterium tuberculosis]
GTGAGAGCATCGCCCACTTCGCCGCCGGAGCAGGTGGTCGTCGACGCGAGTGCCATGGTGGATCTACTGGCTCGCACTAG
CGATCGGTGCTCTGCGGTGCGCGCGCGGCTGGCTCGGACCGCGATGCACGCGCCGGCGCACTTCGATGCAGAGGTGTTGT
CGGCGCTGGGGCGCATGCAGCGCGCCGGCGCACTCACCGTTGCCTATGTCGATGCGGCACTGGAGGAGTTGCGACAGGTG
CCGGTGACTCGACACGGTCTTTCGTCGCTGCTTGCTGGAGCGTGGTCGCGCCGCGACACCCTCCGCCTGACCGATGCCCT
CTACGTCGAGCTGGCCGAAACGGCAGGTCTGGTGTTGTTGACCACCGACGAAAGATTGGCACGCGCCTGGCCCTCGGCTC
ACGCCATCGGCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9543.79 Da
• Isoelectric Point: 4.5994

>AT129500 WP_003402915.1 NZ_CP041792:c645437-645171 [Mycobacterium tuberculosis]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Nucleotide

• Length: 267 bp

>AT129500 NZ_CP041792:c645437-645171 [Mycobacterium tuberculosis]
TTGTTGAGCCGGCGCACGAAAACGATCGTTGTGTGTACATTGGTGTGTATGGCTCGGTTGAACGTGTATGTGCCCGACGA
ATTGGCGGAGCGCGCCAGGGCGCGGGGCTTGAACGTCTCGGCGCTGACTCAGGCCGCGATCAGTGCCGAGTTGGAGAACT
CCGCAACCGATGCGTGGCTTGAGGGGTTGGAACCCAGAAGCACCGGCGCTCGGCATGATGACGTGCTGGGTGCGATCGAT
GCCGCTCGCGATGAGTTCGAAGCGTGA

Structures

Toxin

• AlphaFold DB: G0TPQ9

Antitoxin

• AlphaFold DB: G0TPR0