Detailed information of TA system TA129484

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3074622..3075309
• Replicon: chromosome
• Accession: NZ_CP041791
• Organism: Mycobacterium tuberculosis strain SEA17020023P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: FPK19_RS14405
• Protein ID: WP_003414064.1
• Coordinates: 3074914..3075309 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: FPK19_RS14400
• Protein ID: WP_003414061.1
• Coordinates: 3074622..3074888 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK19_RS14375	3070261..3071163	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
FPK19_RS14380	3071232..3071984	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
FPK19_RS14385	3072228..3072503	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
FPK19_RS14390	3072500..3074122	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
FPK19_RS14395	3074209..3074625	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	-
FPK19_RS14400	3074622..3074888	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPK19_RS14405	3074914..3075309	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPK19_RS14410	3075306..3075575	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
FPK19_RS14415	3075585..3076679	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
FPK19_RS14420	3076676..3077095	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
FPK19_RS14425	3077169..3077648	-	480	WP_003414073.1	dihydrofolate reductase	-
FPK19_RS14430	3077719..3078519	-	801	WP_003911953.1	thymidylate synthase	-
FPK19_RS14435	3078675..3079412	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T129484 WP_003414064.1 NZ_CP041791:c3075309-3074914 [Mycobacterium tuberculosis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T129484 NZ_CP041791:c3075309-3074914 [Mycobacterium tuberculosis]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT129484 WP_003414061.1 NZ_CP041791:c3074888-3074622 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT129484 NZ_CP041791:c3074888-3074622 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64