Detailed information of TA system TA129431

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3713697..3714371
• Replicon: chromosome
• Accession: NZ_CP041790
• Organism: Mycobacterium tuberculosis strain SEA17020024P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FPK20_RS17355
• Protein ID: WP_031666341.1
• Coordinates: 3713697..3714125 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TI59
• Locus tag: FPK20_RS17360
• Protein ID: WP_003417286.1
• Coordinates: 3714129..3714371 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK20_RS17330	3709519..3709920	-	402	WP_003417264.1	cytidine deaminase	-
FPK20_RS17335	3710061..3710495	+	435	WP_072460198.1	succinate dehydrogenase, cytochrome b556 subunit	-
FPK20_RS17340	3710492..3710926	+	435	WP_003417269.1	succinate dehydrogenase hydrophobic membrane anchor subunit	-
FPK20_RS17345	3711055..3712827	+	1773	WP_003417273.1	succinate dehydrogenase flavoprotein subunit	-
FPK20_RS17350	3712827..3713618	+	792	WP_003417279.1	succinate dehydrogenase iron-sulfur subunit	-
FPK20_RS17355	3713697..3714125	-	429	WP_031666341.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPK20_RS17360	3714129..3714371	-	243	WP_003417286.1	ribbon-helix-helix domain-containing protein	Antitoxin
FPK20_RS17365	3714493..3715107	-	615	WP_003417288.1	class I SAM-dependent methyltransferase	-
FPK20_RS17370	3715104..3715769	-	666	WP_003417290.1	molybdopterin converting factor subunit 1	-
FPK20_RS17375	3715770..3716303	-	534	WP_003417293.1	cyclic pyranopterin monophosphate synthase MoaC	-
FPK20_RS17380	3716300..3716674	-	375	WP_003417295.1	4a-hydroxytetrahydrobiopterin dehydratase	-
FPK20_RS17385	3716771..3717835	-	1065	WP_003913037.1	GTP 3',8-cyclase MoaA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-127)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15860.19 Da
• Isoelectric Point: 8.5264

>T129431 WP_031666341.1 NZ_CP041790:c3714125-3713697 [Mycobacterium tuberculosis]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAYAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Nucleotide

• Length: 429 bp

>T129431 NZ_CP041790:c3714125-3713697 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCTATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8849.86 Da
• Isoelectric Point: 5.1007

>AT129431 WP_003417286.1 NZ_CP041790:c3714371-3714129 [Mycobacterium tuberculosis]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Nucleotide

• Length: 243 bp

>AT129431 NZ_CP041790:c3714371-3714129 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Structures

Antitoxin

• AlphaFold DB: G0TI59