Detailed information of TA system TA129421

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2884778..2885415
• Replicon: chromosome
• Accession: NZ_CP041790
• Organism: Mycobacterium tuberculosis strain SEA17020024P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P0DMV7
• Locus tag: FPK20_RS13385
• Protein ID: WP_003413180.1
• Coordinates: 2884778..2885173 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ44
• Locus tag: FPK20_RS13390
• Protein ID: WP_003413183.1
• Coordinates: 2885170..2885415 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK20_RS13345	2880181..2881392	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
FPK20_RS13350	2881519..2882178	+	660	WP_003900847.1	hypothetical protein	-
FPK20_RS13355	2882175..2882837	+	663	WP_003900848.1	hypothetical protein	-
FPK20_RS13360	2882834..2883112	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
FPK20_RS13365	2883205..2883618	+	414	WP_003413164.1	PIN domain nuclease	-
FPK20_RS13370	2883657..2883914	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	-
FPK20_RS13375	2883911..2884288	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	-
FPK20_RS13380	2884304..2884678	-	375	WP_003413177.1	hypothetical protein	-
FPK20_RS13385	2884778..2885173	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	Toxin
FPK20_RS13390	2885170..2885415	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	Antitoxin
FPK20_RS13395	2885826..2886245	-	420	WP_003413190.1	A24 family peptidase	-
FPK20_RS13400	2886257..2887066	-	810	WP_003413193.1	shikimate dehydrogenase	-
FPK20_RS13405	2887063..2888316	-	1254	WP_193018048.1	endolytic transglycosylase MltG	-
FPK20_RS13410	2888309..2888821	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endonuclease VapC20-like (1-129)

Antitoxin

• Ribbon-helix-helix protein, CopG (11-49)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14619.53 Da
• Isoelectric Point: 6.9794

>T129421 WP_003413180.1 NZ_CP041790:c2885173-2884778 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Nucleotide

• Length: 396 bp

>T129421 NZ_CP041790:c2885173-2884778 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9098.33 Da
• Isoelectric Point: 5.6007

>AT129421 WP_003413183.1 NZ_CP041790:c2885415-2885170 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Nucleotide

• Length: 246 bp

>AT129421 NZ_CP041790:c2885415-2885170 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Structures

Toxin

• PDB: 5WZ4
• PDB: 5WZF

Antitoxin

• AlphaFold DB: A0A7U4BW31